Construct: ORF TRCN0000469514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013314.1_s317c1
- Derived from:
- ccsbBroadEn_02557
- DNA Barcode:
- AATCAATCATCTATTCCACCTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GADD45G (10912)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10912 | GADD45G | growth arrest and DNA damag... | NM_006705.4 | 100% | 100% | |
| 2 | human | 10912 | GADD45G | growth arrest and DNA damag... | XM_011518163.2 | 78.6% | 78.6% | 53_54ins102 |
| 3 | mouse | 23882 | Gadd45g | growth arrest and DNA-damag... | NM_011817.2 | 88.2% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 543
- ORF length:
- 477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tctggaagaa gtccgcggcc aggacacagt tccggaaagc acagccagga 121 tgcagggtgc cgggaaagcg ctgcatgagt tgctgctgtc ggcgcagcgt cagggctgcc 181 tcactgccgg cgtcTACGAG TCAGCCAAAG TCTTGAACGT GGACCCCGAC AATGTGACCT 241 TCTGTGTGCT GGCTGCGGGT GAGGAGGACG AGGGCGACAT CGCGCTGCAG ATCCATTTTA 301 CGCTGATCCA GGCTTTCTGC TGCGAGAACG ACATCGACAT AGTGCGCGTG GGCGATGTGC 361 AGCGGCTGGC GGCTATCGTG GGCGCCGGCG AGGAGGCGGG TGCGCCGGGC GACCTGCACT 421 GCATCCTCAT TTCGAACCCC AACGAGGACG CCTGGAAGGA TCCCGCCTTG GAGAAGCTCA 481 GCCTGTTTTG CGAGGAGAGC CGCAGCGTTA ACGACTGGGT GCCCAGCATC ACCCTCCCCG 541 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 601 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 661 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TCAATCATCT ATTCCACCTC CCACGCGTTA 721 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt