Construct: ORF TRCN0000469543
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002016.1_s317c1
- Derived from:
- ccsbBroadEn_04645
- DNA Barcode:
- GGCAAACGCATATTAGATCGGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf57 (113246)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469543
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301834.1 | 100% | 100% | |
| 2 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_138425.4 | 100% | 100% | |
| 3 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301836.1 | 86.1% | 85.7% | (many diffs) |
| 4 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301837.1 | 76.9% | 76.9% | 141_142ins87 |
| 5 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301838.1 | 72.2% | 72.2% | 0_1ins105 |
| 6 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NR_126035.1 | 32.6% | (many diffs) | |
| 7 | mouse | 14790 | Grcc10 | gene rich cluster, C10 gene | NM_013535.1 | 88.6% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 468
- ORF length:
- 378
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGTCCGC CTCGACCCAA CCGGCGGCCT 121 TGAGCGCTGA GCAAGCAAAG GTGGTCCTCG CGGAGGTGAT CCAGGCGTTC TCCGCCCCGG 181 AGAATGCAGT GCGCATGGAC GAGGCTCGGG ATAACGCCTG CAACGACATG GGTAAGATGC 241 TGCAATTCGT GCTGCCCGTG GCCACGCAGA TCCAGCAGGA GGTTATCAAA GCCTATGGCT 301 TCAGCTGCGA CGGGGAAGGT GTCCTTAAGT TTGCTCGCTT GGTCAAGTCC TACGAAGCCC 361 AGGATCCTGA GATCGCCAGC CTGTCAGGCA AGCTGAAGGC GCTGTTTCTG CCGCCCATGA 421 CCCTGCCACC CCATGGGCCT GCTGCTGGTG GCAGCGTGGC CGCCTCCTGC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAGGCAAAC GCATATTAGA TCGGGGTACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt