Construct: ORF TRCN0000469586
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012937.1_s317c1
- Derived from:
- ccsbBroadEn_08063
- DNA Barcode:
- TATCTCAATTCAATATCCCCTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSPH14 (27156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469586
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27156 | RSPH14 | radial spoke head 14 homolog | NM_014433.3 | 99.9% | 100% | 148T>C |
| 2 | human | 27156 | RSPH14 | radial spoke head 14 homolog | XM_011530149.2 | 87.3% | 87.4% | 148T>C;790_939del |
| 3 | human | 27156 | RSPH14 | radial spoke head 14 homolog | XM_011530150.1 | 86.4% | 68.5% | 148T>C;787_919del;1178_1206del |
| 4 | human | 27156 | RSPH14 | radial spoke head 14 homolog | XM_011530151.2 | 86.4% | 68.5% | 148T>C;787_919del;1178_1206del |
| 5 | human | 27156 | RSPH14 | radial spoke head 14 homolog | XM_011530152.2 | 86.4% | 68.5% | 148T>C;787_919del;1178_1206del |
| 6 | human | 27156 | RSPH14 | radial spoke head 14 homolog | XM_017028774.1 | 86.4% | 68.5% | 148T>C;787_919del;1178_1206del |
| 7 | human | 27156 | RSPH14 | radial spoke head 14 homolog | XM_011530154.2 | 69.6% | 52% | 0_1ins204;583_715del;974_1002del |
| 8 | human | 27156 | RSPH14 | radial spoke head 14 homolog | XM_011530155.2 | 52.3% | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1110
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccattcccag aactccttgg agcttcccat taacatcaat gccacccaga 121 ttaccactgc ctatggccat cgggccctgc ccaagctgaa ggaggagctg cagtcagagg 181 acctccagac gaggcagaaa gccctcatgg ccctgtgtga cctcatgcat gaccccgagt 241 gtatctacaa ggccatgaac ataggctgta tggagaacct gaaagctttg ctgaaggata 301 gcaacagtat ggtgcgcata aagaccaccg aggtgctcca catcacggca agccatagcg 361 tgggcagata cgcctttcta gagcacgaca tcgtccttgc cctgtccttc ctgctgaatg 421 accccagccc agtctgccgg gggaacctgt acaaggcata catgcagctg gtccaggtgc 481 ctagaggggc ccaagagatc atcagcaaag gtctgatttc ctcactggta tggaagctgc 541 aggtggaggt ggaggaggag gagttccagg agttcatcct ggacacactg gtcctctgcc 601 tgcaggagga tgccaccgag gccctgggca gcaatgtggt gcttgtcctg aagcagaagc 661 tccTCAGCGC CAACCAGAAC ATCCGCAGCA AGGCCGCCCG TGCGCTCCTT AATGTCAGCA 721 TATCTCGAGA GGGCAAGAAA CAGGTGTGTC ATTTTGACGT CATCCCCATC CTGGTCCATC 781 TGCTGAAAGA CCCAGTGGAG CATGTGAAGT CTAACGCTGC CGGTGCCCTG ATGTTCGCCA 841 CAGTGATCAC TGAAGGGAAG TATGCGGCCC TGGAGGCACA AGCCATCGGC CTGCTCCTGG 901 AGCTGCTGCA CTCCCCCATG ACCATAGCGC GCCTGAATGC CACCAAGGCC CTTACCATGC 961 TGGCAGAGGC CCCCGAGGGC CGCAAGGCCC TGCAGACGCA CGTGCCCACT TTCCGTGCCA 1021 TGGAGGTGGA GACTTACGAA AAGCCTCAAG TGGCCGAAGC CTTACAGCGG GCAGCCCGGA 1081 TCGCCATCAG TGTCATCGAG TTCAAACCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATATCT 1261 CAATTCAATA TCCCCTCATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt