Construct: ORF TRCN0000469604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002867.1_s317c1
- Derived from:
- ccsbBroadEn_02945
- DNA Barcode:
- AAAATAGTTGCTAGAACCGGCGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO9 (26268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469604
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26268 | FBXO9 | F-box protein 9 | NM_033481.3 | 100% | 100% | |
| 2 | human | 26268 | FBXO9 | F-box protein 9 | XM_005248995.4 | 100% | 100% | |
| 3 | human | 26268 | FBXO9 | F-box protein 9 | XM_005248996.4 | 100% | 100% | |
| 4 | human | 26268 | FBXO9 | F-box protein 9 | NM_033480.3 | 92.2% | 92.2% | 1_102del |
| 5 | human | 26268 | FBXO9 | F-box protein 9 | NM_012347.4 | 90.1% | 90.1% | 1_132del |
| 6 | human | 26268 | FBXO9 | F-box protein 9 | XR_001743336.1 | 58.4% | (many diffs) | |
| 7 | mouse | 71538 | Fbxo9 | f-box protein 9 | NM_023605.2 | 81.4% | 85.3% | (many diffs) |
| 8 | mouse | 71538 | Fbxo9 | f-box protein 9 | NM_001081490.2 | 81.2% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatgtt ccgagctcag tggatgtttg aacttgctcc aggtgtaagc tctagcaatt 121 tagaaaatcg accttgcaga gcagcaagag gctctctcca gaaaacatcg gcagatacca 181 aaggaaaaca agaacaggca aaagaagaaa aggctcgaga actcttccta aaagcagtag 241 aagaagaaca aaatggagct ctctatgaag ccatcaagtt ttatcgtagg gctatgcaac 301 ttgtacctga tatagagttc aagattactt atacccggtc tccagatggt gatggcgttg 361 gaaacagcta cattgaagat aatgatgatg acagcaaaat ggcagatctc ttgtcctact 421 tccagcagca actcacattt caggagtctg tgcttaaact gtgtcagcct gagcttgaga 481 gcagtcagat tcacatatca gtgctgccaa tggaggtcct gatgtacatc ttccgatggg 541 tggtgtctag tgacttggac ctcagatcat tggagcagtt gtcgctggtg tgcagaggat 601 tctacatctg tgccagagac cctgaaatat ggcgtctggc ctgcttgaaa gtttggggca 661 gaagctgtat taaacttgtt ccgtacacgt cctggagaga gatgttttta gaacggcctc 721 gtgttcggtt tgatggcgtg tatatcagta aaaccacata tattcgtcaa ggggaacagt 781 ctcttgatgg tttctataga gcctggcacc aagtggaata ttacaggtac ataagattct 841 ttcctgatgg ccatgtgatg atgttgacaa cccctgaaga gcctcagtcc attgttccac 901 gtttaagaac taggaatacc aggactgatg caattcTACT GGGTCACTAT CGCTTGTCAC 961 AAGACACAGA CAATCAGACC AAAGTATTTG CTGTAATAAC TAAGAAAAAA GAAGAAAAAC 1021 CACTTGACTA TAAATACAGA TATTTTCGTC GTGTCCCTGT ACAAGAAGCA GATCAGAGTT 1081 TTCATGTGGG GCTACAGCTA TGTTCCAGTG GTCACCAGAG GTTCAACAAA CTCATCTGGA 1141 TACATCATTC TTGTCACATT ACTTACAAAT CAACTGGTGA GACTGCAGTC AGTGCTTTTG 1201 AGATTGACAA GATGTACACC CCCTTGTTCT TCGCCAGAGT AAGGAGCTAC ACAGCTTTCT 1261 CAGAAAGGCC TCTGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAAATAGTTG CTAGAACCGG 1441 CGCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt