Construct: ORF TRCN0000469612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006435.1_s317c1
- Derived from:
- ccsbBroadEn_10881
- DNA Barcode:
- CAGTTAAGCTATTAAATCGTGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HLA-DQB2 (3120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3120 | HLA-DQB2 | major histocompatibility co... | NM_001198858.2 | 97.5% | 97.8% | (many diffs) |
| 2 | human | 3120 | HLA-DQB2 | major histocompatibility co... | XM_011514561.3 | 94.2% | 94.3% | (many diffs) |
| 3 | human | 3120 | HLA-DQB2 | major histocompatibility co... | XM_011514560.2 | 85.8% | 84.3% | (many diffs) |
| 4 | human | 3120 | HLA-DQB2 | major histocompatibility co... | NM_001300790.2 | 84% | 84.3% | (many diffs) |
| 5 | human | 3120 | HLA-DQB2 | major histocompatibility co... | XM_005249051.4 | 80.1% | 79.9% | (many diffs) |
| 6 | human | 3119 | HLA-DQB1 | major histocompatibility co... | NM_001243962.1 | 79.2% | 71.6% | (many diffs) |
| 7 | human | 3119 | HLA-DQB1 | major histocompatibility co... | NM_002123.5 | 76.2% | 67.4% | (many diffs) |
| 8 | human | 3119 | HLA-DQB1 | major histocompatibility co... | NM_001243961.2 | 75.6% | 67.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 759
- ORF length:
- 693
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttggaagatg gctctgcaga tccctggagg cttttgggca gcagctgtga 121 ccgtgatgct ggtgatgctg agcaccccag tggctgaggc cagagacttt cccaaggatt 181 tcttggtcca gtttaagggc atgtgctact tcaccaacgg gacagagcgc gtgcggggtg 241 tggccagata catctataac cgcgaggagt acgggcgctt cgacagcGAC GTTGGGGAGT 301 TCCAGGCGGT GACCGAGCTG GGGCGGAGCA TCGAGGACTG GAACAACTAT AAGGACTTCT 361 TGGAGCAGGA GCGGGCCGCG GTGGACAAGG TGTGCAGACA CAACTACGAG GCGGAGCTAC 421 GCACGACCTT GCAGCGGCAA GTGGAGCCCA CAGTGACCAT CTCCCCATCC AGGACAGAGG 481 CCCTCAACCA CCACAACCTG CTGGTCTGCT CAGTGACAGA TTTCTATCCA GCCCAGATCA 541 AAGTCCAGTG GTTTCGGAAT GACCAGGAGG AGACAGCCGG TGTTGTGTCC ACCTCCCTCA 601 TTAGGAATGG TGACTGGACC TTCCAGATTC TGGTGATGCT GGAAATAACT CCCCAGCGTG 661 GAGACATCTA CACCTGCCAA GTGGAGCACC CCAGCCTCCA GAGCCCCATC ACCGTGGAGT 721 GGCGACCTCG AGGGCCTCCA CCTGCAGGAC TCCTGCACTG CCCAACTTTC TTGTACAAAG 781 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 841 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 901 ACGACAGTTA AGCTATTAAA TCGTGGCTAC GCGTTAAGTC gacaatcaac ctctggatta 961 caaaatttgt gaaagatt