Construct: ORF TRCN0000469644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009670.1_s317c1
- Derived from:
- ccsbBroadEn_11624
- DNA Barcode:
- TCGAAATAAGCCCCTAAACTCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCRG1 (11341)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11341 | SCRG1 | stimulator of chondrogenesis 1 | NM_001329597.2 | 60.2% | 60.2% | 178_294del |
| 2 | human | 11341 | SCRG1 | stimulator of chondrogenesis 1 | NM_007281.4 | 60.2% | 60.2% | 178_294del |
| 3 | human | 11341 | SCRG1 | stimulator of chondrogenesis 1 | XM_024453882.1 | 60.2% | 60.2% | 178_294del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 213
- ORF end:
- 390
- ORF length:
- 177
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGAAACT GATGGTACTT GTTTTCACCA 241 TTGGGCTAAC TTTGCTGCTA GGAGTTCAAG CCATGCCTGC AAATCGCCTC TCTTGCTACA 301 GAAAGATACT AAAAGATCAC AACTGTCACA ACCTTCCGGA AGGAGTAGCT GACCTGACAC 361 AGATTGATGT CAATGTCCAG GATCATTTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCGAA 541 ATAAGCCCCT AAACTCACGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt