Construct: ORF TRCN0000469661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018094.1_s317c1
- Derived from:
- ccsbBroadEn_02680
- DNA Barcode:
- CGGCCTGAATTTAAGGTTTGGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OIP5 (11339)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11339 | OIP5 | Opa interacting protein 5 | NM_007280.2 | 100% | 100% | |
| 2 | human | 11339 | OIP5 | Opa interacting protein 5 | NM_001317860.1 | 82% | 82% | 388_389ins123 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctcagccg ctgcggcatc gctcacgttg tgcaacgccg ccccgggggg 121 acttttgtgg tggcactgag agggcgattg accaagcttc ttttacgacc tccatggagt 181 gggatacgca ggtggtgaag gggtcctcgc cgctcggccc cgcagggctg ggggctgagg 241 agccagccgc cggcccgcag ctgccgtctt ggctgcagcc tgagaggtgc gctgtgttcc 301 agtgcgcaca gtgtcacgca gtgctcgccg actcggtgca cctcgcctgg gacctgtcgc 361 ggtccctcgg ggccgtggTC TTCTCCAGAG TTACAAATAA CGTCGTTTTG GAAGCGCCCT 421 TCCTAGTTGG CATTGAAGGT TCACTCAAAG GCAGTACTTA CAACCTTTTA TTCTGTGGTT 481 CTTGTGGGAT TCCCGTTGGT TTCCATCTGT ATTCTACCCA TGCTGCCCTG GCTGCCTTGA 541 GAGGTCACTT CTGCCTTTCC AGTGACAAAA TGGTGTGCTA TCTCTTAAAA ACAAAAGCCA 601 TAGTAAATGC ATCAGAGATG GATATTCAAA ATGTTCCTCT ATCAGAAAAG ATTGCAGAGC 661 TGAAAGAGAA GATAGTGCTA ACGCACAATC GCTTAAAATC ACTAATGAAG ATTCTGAGTG 721 AAGTGACTCC TGACCAGTCC AAGCCAGAAA ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 901 GCCTGAATTT AAGGTTTGGT GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt