Construct: ORF TRCN0000469668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000377.1_s317c1
- Derived from:
- ccsbBroadEn_09285
- DNA Barcode:
- GCTGACCAGAAACTCTGACTATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LACRT (90070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469668
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90070 | LACRT | lacritin | NM_033277.2 | 99.7% | 100% | 219G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa atttaccact ctcctcttct tggcagctgt agcaggggcc ctggtctatg 121 ctgaagatgc ctccTCTGAC TCGACGGGTG CTGATCCTGC CCAGGAAGCT GGGACCTCTA 181 AGCCTAATGA AGAGATCTCA GGTCCAGCAG AACCAGCTTC ACCCCCAGAG ACAACCACAA 241 CAGCCCAGGA GACTTCGGCG GCAGCAGTTC AGGGGACAGC CAAAGTCACC TCAAGCAGGC 301 AGGAACTAAA CCCCCTGAAA TCCATAGTGG AGAAAAGTAT CTTACTAACA GAACAAGCCC 361 TTGCAAAAGC AGGAAAAGGA ATGCACGGAG GCGTGCCAGG TGGAAAACAA TTCATCGAAA 421 ATGGAAGTGA ATTTGCACAA AAATTACTGA AGAAATTCAG TCTATTAAAA CCATGGGCAT 481 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGAGCTGA CCAGAAACTC TGACTATTTA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt