Construct: ORF TRCN0000469668

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000377.1_s317c1
Derived from:
ccsbBroadEn_09285
DNA Barcode:
GCTGACCAGAAACTCTGACTATTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LACRT (90070)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469668
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 90070 LACRT lacritin NM_033277.2 99.7% 100% 219G>A
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
480
ORF length:
414
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa atttaccact ctcctcttct tggcagctgt agcaggggcc ctggtctatg
121ctgaagatgc ctccTCTGAC TCGACGGGTG CTGATCCTGC CCAGGAAGCT GGGACCTCTA
181AGCCTAATGA AGAGATCTCA GGTCCAGCAG AACCAGCTTC ACCCCCAGAG ACAACCACAA
241CAGCCCAGGA GACTTCGGCG GCAGCAGTTC AGGGGACAGC CAAAGTCACC TCAAGCAGGC
301AGGAACTAAA CCCCCTGAAA TCCATAGTGG AGAAAAGTAT CTTACTAACA GAACAAGCCC
361TTGCAAAAGC AGGAAAAGGA ATGCACGGAG GCGTGCCAGG TGGAAAACAA TTCATCGAAA
421ATGGAAGTGA ATTTGCACAA AAATTACTGA AGAAATTCAG TCTATTAAAA CCATGGGCAT
481GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
541GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
601TTTATATATC TTGTGGAAAG GACGAGCTGA CCAGAAACTC TGACTATTTA CGCGTTAAGT
661Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)