Construct: ORF TRCN0000469677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011099.1_s317c1
- Derived from:
- ccsbBroadEn_01350
- DNA Barcode:
- AGATTCCCTGAACCCCTTAGAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECTIN2 (5819)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5819 | NECTIN2 | nectin cell adhesion molecu... | NM_002856.3 | 100% | 100% | |
| 2 | human | 5819 | NECTIN2 | nectin cell adhesion molecu... | NM_001042724.2 | 78.2% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1503
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgggccgct gccctcctgc cgtcgagatc gccgccgacg ccgctgctgt 121 ggccgctgct gctgctgctg ctcctggaaa ccggagccca ggatgtgcga gttcaagtgc 181 tacccgaggt gcgaggccag ctcgggggca ccgtggagct gccgtgccac ctgctgccac 241 ctgttcctgg actgtacatc tccctggtga cctggcagcg cccagatgca cctgcgaacc 301 accagaatgt ggccgccttc caccctaaga tgggtcccag cttccccagc ccgaagcctg 361 gcagcgagcg gctgtccttc gtctctgcca agcagagcac tgggcaagac acagaggcag 421 agctccagga cgccacgctg gccctccacg ggctcacggt ggaggacgag ggcaactaca 481 cttgcgagtt tgccaccttc cccaaggggt ccgtccgagg gatgacctgg ctcagagtca 541 tagccaagcc caagaaccaa gctgaggccc agaaggtcac gttcagccag gaccctacga 601 cagtggccct ctgcatctcc aaagagggcc gcccacctgc ccggatctcc tggctctcat 661 ccctggactg ggaagccaaa gagactcagg tgtcagggac cctggccgga actgtcactg 721 tcaccagccg cttcaccttg gtgccctcgg gccgagcaga tggtgtcacg gtcacctgca 781 aagtggagca tgagagcttc gaggaaccag ccctgatacc tgtgaccctc tctgtacgct 841 accctcctga agtgtccatc tccggctatg atgacaactg gtacctcggc cgtactgatg 901 ccaccctgag ctgtgacgtc cgcagcaacc cagagcccac gggctatgac tggagcacga 961 cctcaggcac cttcccgacc tccgcagtgg cccagggctc ccagctggtc atccacgcag 1021 tggacagtct gttcaatacc accTTCGTCT GCACAGTCAC CAATGCCGTG GGCATGGGCC 1081 GCGCTGAGCA GGTCATCTTT GTCCGAGAAA CCCCCAGGGC CTCGCCCCGA GATGTGGGCC 1141 CGCTGGTGTG GGGGGCCGTG GGGGGGACAC TGCTGGTGCT GCTGCTTCTG GCTGGGGGGT 1201 CCTTGGCCTT CATCCTGCTG AGGGTGAGGA GGAGGAGGAA GAGCCCTGGA GGAGCAGGAG 1261 GAGGAGCCAG TGGCGACGGG GGATTCTACG ATCCGAAAGC TCAGGTGTTG GGAAATGGGG 1321 ACCCCGTCTT CTGGACACCA GTAGTCCCTG GTCCCATGGA ACCAGATGGC AAGGATGAGG 1381 AGGAGGAGGA GGAGGAAGAG AAGGCAGAGA AAGGCCTCAT GTTGCCTCCA CCCCCAGCAC 1441 TCGAGGATGA CATGGAGTCC CAGCTGGACG GCTCCCTCAT CTCACGGCGG GCAGTTTATG 1501 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGAAG ATTCCCTGAA CCCCTTAGAA CCACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt