Construct: ORF TRCN0000469677

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011099.1_s317c1
Derived from:
ccsbBroadEn_01350
DNA Barcode:
AGATTCCCTGAACCCCTTAGAACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NECTIN2 (5819)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469677
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5819 NECTIN2 nectin cell adhesion molecu... NM_002856.3 100% 100%
2 human 5819 NECTIN2 nectin cell adhesion molecu... NM_001042724.2 78.2% 70.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1503
ORF length:
1437
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ccgggccgct gccctcctgc cgtcgagatc gccgccgacg ccgctgctgt
121ggccgctgct gctgctgctg ctcctggaaa ccggagccca ggatgtgcga gttcaagtgc
181tacccgaggt gcgaggccag ctcgggggca ccgtggagct gccgtgccac ctgctgccac
241ctgttcctgg actgtacatc tccctggtga cctggcagcg cccagatgca cctgcgaacc
301accagaatgt ggccgccttc caccctaaga tgggtcccag cttccccagc ccgaagcctg
361gcagcgagcg gctgtccttc gtctctgcca agcagagcac tgggcaagac acagaggcag
421agctccagga cgccacgctg gccctccacg ggctcacggt ggaggacgag ggcaactaca
481cttgcgagtt tgccaccttc cccaaggggt ccgtccgagg gatgacctgg ctcagagtca
541tagccaagcc caagaaccaa gctgaggccc agaaggtcac gttcagccag gaccctacga
601cagtggccct ctgcatctcc aaagagggcc gcccacctgc ccggatctcc tggctctcat
661ccctggactg ggaagccaaa gagactcagg tgtcagggac cctggccgga actgtcactg
721tcaccagccg cttcaccttg gtgccctcgg gccgagcaga tggtgtcacg gtcacctgca
781aagtggagca tgagagcttc gaggaaccag ccctgatacc tgtgaccctc tctgtacgct
841accctcctga agtgtccatc tccggctatg atgacaactg gtacctcggc cgtactgatg
901ccaccctgag ctgtgacgtc cgcagcaacc cagagcccac gggctatgac tggagcacga
961cctcaggcac cttcccgacc tccgcagtgg cccagggctc ccagctggtc atccacgcag
1021tggacagtct gttcaatacc accTTCGTCT GCACAGTCAC CAATGCCGTG GGCATGGGCC
1081GCGCTGAGCA GGTCATCTTT GTCCGAGAAA CCCCCAGGGC CTCGCCCCGA GATGTGGGCC
1141CGCTGGTGTG GGGGGCCGTG GGGGGGACAC TGCTGGTGCT GCTGCTTCTG GCTGGGGGGT
1201CCTTGGCCTT CATCCTGCTG AGGGTGAGGA GGAGGAGGAA GAGCCCTGGA GGAGCAGGAG
1261GAGGAGCCAG TGGCGACGGG GGATTCTACG ATCCGAAAGC TCAGGTGTTG GGAAATGGGG
1321ACCCCGTCTT CTGGACACCA GTAGTCCCTG GTCCCATGGA ACCAGATGGC AAGGATGAGG
1381AGGAGGAGGA GGAGGAAGAG AAGGCAGAGA AAGGCCTCAT GTTGCCTCCA CCCCCAGCAC
1441TCGAGGATGA CATGGAGTCC CAGCTGGACG GCTCCCTCAT CTCACGGCGG GCAGTTTATG
1501TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1561TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1621GGCTTTATAT ATCTTGTGGA AAGGACGAAG ATTCCCTGAA CCCCTTAGAA CCACGCGTTA
1681AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)