Construct: ORF TRCN0000469685

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005159.1_s317c1
Derived from:
ccsbBroadEn_07425
DNA Barcode:
AATATTCTTTATACGACCAGCCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STX8 (9482)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469685
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9482 STX8 syntaxin 8 NM_004853.3 99.7% 100% 94A>C;456C>T
2 human 9482 STX8 syntaxin 8 XR_934120.2 69% (many diffs)
3 human 9482 STX8 syntaxin 8 XM_011524079.2 64.4% 60.4% (many diffs)
4 human 9482 STX8 syntaxin 8 NR_033656.1 50.6% (many diffs)
5 mouse 55943 Stx8 syntaxin 8 NM_018768.2 88.5% 92.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
774
ORF length:
708
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc accggacccc tggttctcca catacgattc tacttgtcaa attgcccaag
121aaattgctga gaaaattcaa caacgaaatc aatatgaacg aaaaggtgaa aaggcaccaa
181agcttaccgt gacaatcaga gctttgttgc agaacctgaa ggaaaagatc gcccttttga
241aggacttatt gctaagagct gtgtcaacac atcagataac acagcttgaa ggggaccgaa
301gacagaacct cttGGATGAT CTTGTAACTC GAGAGAGACT ACTTCTGGCA TCCTTTAAGA
361ATGAGGGTGC CGAACCAGAT CTAATCAGGT CCAGCCTGAT GAGTGAAGAG GCTAAGCGAG
421GAGCACCCAA CCCTTGGCTC TTTGAGGAGC CAGAGGAGAC CAGAGGCTTG GGTTTTGATG
481AAATCCGGCA ACAGCAGCAG AAAATTATCC AAGAACAGGA TGCAGGCCTT GATGCCCTTT
541CCTCTATCAT AAGTCGCCAA AAACAAATGG GGCAGGAAAT TGGGAATGAA TTGGATGAAC
601AAAATGAGAT AATTGACGAC CTTGCCAACC TAGTGGAGAA CACAGATGAA AAACTTCGCA
661ATGAAACCAG GCGGGTAAAC ATGGTGGACA GAAAGTCAGC CTCTTGTGGG ATGATCATGG
721TGATTTTACT GCTGCTTGTG GCTATCGTGG TTGTTGCAGT CTGGCCGACC AACTGCCCAA
781CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
841ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
901TATCTTGTGG AAAGGACGAA ATATTCTTTA TACGACCAGC CATACGCGTT AAGTCgacaa
961tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)