Construct: ORF TRCN0000469685
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005159.1_s317c1
- Derived from:
- ccsbBroadEn_07425
- DNA Barcode:
- AATATTCTTTATACGACCAGCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX8 (9482)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469685
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9482 | STX8 | syntaxin 8 | NM_004853.3 | 99.7% | 100% | 94A>C;456C>T |
| 2 | human | 9482 | STX8 | syntaxin 8 | XR_934120.2 | 69% | (many diffs) | |
| 3 | human | 9482 | STX8 | syntaxin 8 | XM_011524079.2 | 64.4% | 60.4% | (many diffs) |
| 4 | human | 9482 | STX8 | syntaxin 8 | NR_033656.1 | 50.6% | (many diffs) | |
| 5 | mouse | 55943 | Stx8 | syntaxin 8 | NM_018768.2 | 88.5% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc accggacccc tggttctcca catacgattc tacttgtcaa attgcccaag 121 aaattgctga gaaaattcaa caacgaaatc aatatgaacg aaaaggtgaa aaggcaccaa 181 agcttaccgt gacaatcaga gctttgttgc agaacctgaa ggaaaagatc gcccttttga 241 aggacttatt gctaagagct gtgtcaacac atcagataac acagcttgaa ggggaccgaa 301 gacagaacct cttGGATGAT CTTGTAACTC GAGAGAGACT ACTTCTGGCA TCCTTTAAGA 361 ATGAGGGTGC CGAACCAGAT CTAATCAGGT CCAGCCTGAT GAGTGAAGAG GCTAAGCGAG 421 GAGCACCCAA CCCTTGGCTC TTTGAGGAGC CAGAGGAGAC CAGAGGCTTG GGTTTTGATG 481 AAATCCGGCA ACAGCAGCAG AAAATTATCC AAGAACAGGA TGCAGGCCTT GATGCCCTTT 541 CCTCTATCAT AAGTCGCCAA AAACAAATGG GGCAGGAAAT TGGGAATGAA TTGGATGAAC 601 AAAATGAGAT AATTGACGAC CTTGCCAACC TAGTGGAGAA CACAGATGAA AAACTTCGCA 661 ATGAAACCAG GCGGGTAAAC ATGGTGGACA GAAAGTCAGC CTCTTGTGGG ATGATCATGG 721 TGATTTTACT GCTGCTTGTG GCTATCGTGG TTGTTGCAGT CTGGCCGACC AACTGCCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAA ATATTCTTTA TACGACCAGC CATACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att