Construct: ORF TRCN0000469692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010307.1_s317c1
- Derived from:
- ccsbBroadEn_00064
- DNA Barcode:
- GCTGCTGGAGACCATGCACCTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMPD3 (272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 272 | AMPD3 | adenosine monophosphate dea... | NM_000480.3 | 100% | 100% | |
| 2 | human | 272 | AMPD3 | adenosine monophosphate dea... | NM_001025390.2 | 99.2% | 99.1% | (many diffs) |
| 3 | human | 272 | AMPD3 | adenosine monophosphate dea... | NM_001025389.2 | 98.8% | 98.8% | 0_1ins27 |
| 4 | human | 272 | AMPD3 | adenosine monophosphate dea... | NM_001172430.1 | 98.8% | 98.8% | 0_1ins27 |
| 5 | human | 272 | AMPD3 | adenosine monophosphate dea... | NM_001172431.1 | 78.3% | 78.3% | 0_1ins504 |
| 6 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XM_006507221.3 | 88% | 94.2% | (many diffs) |
| 7 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XM_006507215.3 | 87.6% | 93.4% | (many diffs) |
| 8 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | NM_001276301.1 | 87% | 93.2% | (many diffs) |
| 9 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | NM_009667.3 | 87% | 93.2% | (many diffs) |
| 10 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XM_006507222.3 | 87% | 93.2% | (many diffs) |
| 11 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XM_006507219.3 | 83.2% | 88.9% | (many diffs) |
| 12 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XM_006507217.3 | 81% | 85.9% | (many diffs) |
| 13 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XR_378209.3 | 72.5% | (many diffs) | |
| 14 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XM_017321937.1 | 50.9% | 55.9% | (many diffs) |
| 15 | mouse | 11717 | Ampd3 | adenosine monophosphate dea... | XM_017321938.1 | 50.9% | 55.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2397
- ORF length:
- 2328
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctgtcg tccgaacccg ctgagatgcc gcggcagttt cccaagctga 121 acatctctga agtggatgag caagtccggc tcctggcgga gaaggtgttt gctaaagtgc 181 tccgagaaga ggacagcaaa gatgccctgt ccctgttcac tgtcccagag gactgcccca 241 tcgggcaaaa ggaagccaag gagagggagc tgcagaagga gctggcagag cagaagtctg 301 tggagaccgc aaaaagaaag aaaagtttca agatgattcg gtcccagtcc ctgtctctgc 361 aaatgccgcc acagcaagat tggaagggcc ccccggcagc cagtccggcc atgtctccca 421 caacccctgt ggtcactgga gccacttccc tgcccacgcc agcaccctat gccatgcctg 481 agttccagcg ggtcaccatc agcggagatt actgtgccgg gatcactttg gaggactatg 541 agcaggcagc caagagtctg gccaaggccc taatgatccg ggagaagtat gcgcggctcg 601 cctaccaccg cttcccgcgg atcacatccc agtacctggg tcatccgcgg gcggatactg 661 cacctccgga agagggcctt ccagacttcc accctcctcc actgccccag gaagacccct 721 actgcctgga tgatgcaccc cccaacctgg attacttggt ccacatgcag gggggcatcc 781 tctttgtgta tgataacaag aagatgctgg agcaccagga gccgcacagc ctaccctacc 841 ccgacctgga gacctacacg gtggacatga gccacatcct ggctctcatc accgatggcc 901 ccacgaaaac ctattgtcac cggcgactga actttctgga atccaagttc agccttcatg 961 agatgttaaa cgaaatgtcc gagttcaaag agttgaagag taacccccac cgggacttct 1021 ataacgtgag aaaggtggac acacacatcc atgcggccgc ctgcatgaac caaaagcatc 1081 tgctgcgctt catcaagcac acataccaga cggagcctga caggactgtg gcagagaagc 1141 ggggccggaa gatcaccctg cggcaggtgt ttgacggcct gcacatggac ccctacgacc 1201 tcactgtgga ctcactggat gtccacgcgg gccggcagac attccaccgc tttgacaagt 1261 tcaactccaa atacaaccct gtgggggcca gtgagctgcg tgacctgtat ttgaaaactg 1321 aaaactatct gggaggagag tactttgctc ggatggtcaa ggaggttgcc cgggagctgg 1381 aggagagcaa gtaccagtac tcagagccac ggctctccat ctacggccgc agtcctgagg 1441 agtggcccaa cctggcctac tggttcatcc agcacaaggt ctactctccc aacatgcgct 1501 ggatcatcca ggtgccccgg atttatgaca tatttaggtc aaagaagctg ctgccaaact 1561 ttgggaagat gctggagaac atcttcctgc cccttttcaa ggccactatc aacccccaag 1621 atcatcgaga gcttcacctc ttccttaaat atgtgacggg gtttgacagc gtggatgatg 1681 agtccaagca cagcgaccac atgttttccg acaagagccc aaacccggac gtctggacca 1741 gtgagcagaa cccaccctac agctactacc tgtactacat gtatgccaac atcatggtgc 1801 tcaacaacct ccgcagggag cgcggcctga gcacgttcct gttccggccg cactgtgggg 1861 aagccggctc catcacccac ctggtgtctg ccttcctcac tgctgacaac atttcccacg 1921 ggctgctcct caagaagagt ccggtattgc agtatctcta ctaccttgct cagatcccca 1981 ttgccatgtc tccTCTTAGC AACAACAGTT TGTTCCTCGA ATATTCCAAG AACCCTCTGA 2041 GGGAATTCCT ACACAAGGGA CTGCATGTTT CTCTTTCCAC CGATGACCCC ATGCAGTTCC 2101 ACTACACGAA GGAAGCACTT ATGGAAGAAT ATGCCATTGC AGCTCAAGTG TGGAAGCTGA 2161 GCACCTGCGA CCTGTGTGAG ATCGCCAGGA ACAGCGTGCT GCAGAGCGGC CTCTCGCATC 2221 AGGAAAAGCA AAAGTTTCTG GGACAAAATT ATTATAAAGA AGGACCTGAA GGAAATGATA 2281 TTCGAAAGAC AAATGTGGCT CAGATCCGGA TGGCATTCCG ATATGAGACC TTATGCAATG 2341 AGCTCAGCTT CCTGTCTGAT GCTATGAAAT CAGAAGAGAT CACCGCCTTG ACCAACTTGC 2401 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2461 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2521 ATATATCTTG TGGAAAGGAC GAGCTGCTGG AGACCATGCA CCTTAGACGC GTTAAGTCga 2581 caatcaacct ctggattaca aaatttgtga aagatt