Construct: ORF TRCN0000469710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012054.1_s317c1
- Derived from:
- ccsbBroadEn_09648
- DNA Barcode:
- CAATATGTTAACCATCCATATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF548 (147694)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469710
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147694 | ZNF548 | zinc finger protein 548 | NM_152909.3 | 99.8% | 99.6% | 172G>T;217G>A |
| 2 | human | 147694 | ZNF548 | zinc finger protein 548 | XM_017026316.1 | 99% | 98.5% | (many diffs) |
| 3 | human | 147694 | ZNF548 | zinc finger protein 548 | NM_001172773.2 | 97.6% | 96.8% | 14_49del;208G>T;253G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1665
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cctgactgag ggccgtgtgg tctttgagga cgtggccata tatttctccc 121 aggaggagtg ggggcacctt gatgaggctc agagattgct gtaccgtgat gtgatgctgg 181 agaatttggc ccttttgtcc tcactaggtt cttggcatgg agctgaggat gaggagtcac 241 cttcacagca aggtttttct gtaggagtgt cagaggttac aacttcaaag ccctgtctgt 301 ccagccagaa ggtccaccct agtgagacat gtggcccacc cttgaaagac attctgtgcc 361 tggttgagca caatggaatt catcctgagc aacacatata tatttgtgag gcagagcttt 421 ttcagcaccc aaagcagcaa attggagaaa atctttccag aggggatgat tggatacctt 481 catttgggaa gaaccacaga gttcacatgg cagaggagat cttcacatgc atggagggct 541 ggaaggactt accagccacc tcatgccttc tccagcacca gggccctcaa agcgagtgga 601 agccatacag ggacacagag gacagagaag cctttcagac tggacaaaat gattacaaat 661 gtagtgaatg tgggaaaacc ttcacctgca gctattcatt tgttgagcac cagaaaatcc 721 acacaggaga aaggtcttat gaatgtaaca aatgtgggaa attctttaag tacagtgcca 781 atttcatgaa acatcagaca gttcacacta gtgaaaggac ttatgagtgc agagaatgtg 841 gaaaatcctt tatgtacaac taccgactca tgagacataa gcgagttcac actggagaaa 901 ggccttatga gtgcaacaca tgtgggaaat tctttcggta cagctccaca tttgttagac 961 atcagagagt tcacaccgga gaaaggccgt atgagtgcag ggaatgtggg aaattcttta 1021 tggacagctc cacactcatt aaacatcaga gagttcacac cggagaaaga ccttataagt 1081 gcaatgattg tgggaaattt tttaggtata tctccacact cattagacat cagagaattc 1141 acactggaga aaggccttat gagtgcagtg tatgtgggga attgtttagg tacaactcca 1201 gccttgttaa acattggaga aatcacactg gagaaaggCC TTATAAATGC AGTGAATGTG 1261 GGAAATCATT TAGGTACCAC TGCAGGCTCA TTAGACACCA GAGAGTCCAC ACGGGAGAAA 1321 GGCCTTATGA GTGCAGCGAA TGCGGGAAAT TCTTTCGTTA CAACTCCAAC CTCATTAAAC 1381 ATTGGAGAAA TCACACTGGA GAAAGGCCTT ACGAGTGCAG AGAGTGTGGG AAAGCCTTTA 1441 GCCACAAGCA TATACTTGTT GAGCACCAGA AAATCCACAG TGGAGAAAGA CCTTATGAGT 1501 GCAGCGAATG CCAGAAGGCC TTTATTAGAA AGTCTCACCT GGTTCATCAC CAGAAAATCC 1561 ACAGTGAAGA GAGGCTTGTG TGCTCCATGA ATGTGGGGAA TTCTTTAGCT AAAACTCCAA 1621 CCTCATTAAA CATCAGAGAT TTCACAATGG AGAAAGTTTA CCATTGCCCA ACTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA CAATATGTTA ACCATCCATA TTTCACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt