Construct: ORF TRCN0000469732
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007048.1_s317c1
- Derived from:
- ccsbBroadEn_06072
- DNA Barcode:
- GGTTTCAAGCCTGTGTATCACCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2A7 (1549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469732
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1549 | CYP2A7 | cytochrome P450 family 2 su... | NM_000764.2 | 99.5% | 98.5% | (many diffs) |
| 2 | human | 1548 | CYP2A6 | cytochrome P450 family 2 su... | NM_000762.6 | 96.4% | 93.9% | (many diffs) |
| 3 | human | 1553 | CYP2A13 | cytochrome P450 family 2 su... | NM_000766.5 | 94.6% | 92.1% | (many diffs) |
| 4 | human | 1549 | CYP2A7 | cytochrome P450 family 2 su... | NM_030589.2 | 89.2% | 88% | (many diffs) |
| 5 | human | 1549 | CYP2A7 | cytochrome P450 family 2 su... | XR_935754.3 | 25.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1551
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggcctca gggctgcttc tggtggcctt gctggcctgc ctgactgtga 121 tggtcttgat gtctgtctgg cagcagagga agagcagggg gaagctgcct ccgggaccca 181 ccccactgcc cttcattgga aactacctcc agctgaacac agagcacata tgtgactcca 241 tcatgaagtt cagtgagtgc tatggccccg tgttcaccat tcacttgggg ccccggcggg 301 ccgtggtgct gtgtggacat gatgccgtca gggaggctct ggtggaccag gctgaggagt 361 tcagcgggcg aggcgagcaa gccaccttcg actgggtctt caaaggctat ggcgtggcgt 421 tcagcaacgg ggagcgcgcc aagcagctcc tgcgctttgc catcgccacc ctgagggact 481 tcggggtggg caagcgaggc atcgaggagc gcatccagga ggagtcgggc ttcctcatcg 541 aggccatccg gagcacgcac ggcgccaata tcgatcccac cttcttcctg agccgcacag 601 tctccaatgt catcagctcc attgtctttg gggaccgctt tgactatgag gacaaagagt 661 tcctgtcact gctgagcgtg atgctaggaa tcttccagtt cacgtcaacc tccacggggc 721 agctctatga gatgttctct tcggtgatga aacacctgcc aggaccacag caacaggcct 781 ttaagttgct gcaagggctg gaggacttca tagccaagaa ggtggagcac aaccagcgca 841 cgctggatcc caattcccca cgggacttca tcgactcctt tctcatccgc atgcaggagg 901 aggagaagaa ccccaacacg gagttctact tgaagaacct gatgatgagc acgttgaacc 961 tcttcattgc aggcaccgag acggtcagca ccaccctgcg ctatggcttc ttgctgctca 1021 tgaagcaccc agaggtggag gccaaggtcc atgaggagat tgacagagtg atcggcaaga 1081 accggcagcc caagtttgag gaccgggcca agatgcccta catggaggca gtgatccacg 1141 agatccaaag atttGGAGAC GTGATCCCCA TGAGTTTGGC CCGCAGGGTT AAAAAGGACA 1201 CCAAGTTTCG GGATTTTTTC CTCCCTAAGG GCACCGAAGT GTTCCCTATG CTGGGCTCCG 1261 TGCTGAGAGA CCCCAGCTTC TTCTCCAACC CTCAGGACTT CAATCCCCAG CATTTCCTGG 1321 ATGACAAGGG GCAGTTTAAG AAGAGTGATG CTTTTGTGCC CTTTTCCATC GGAAAGCGGT 1381 ACTGTTTCGG AGAAGGCCTG GCCAGAATGG AGCTCTTTCT CTTCTTCACC ACCGTCATGC 1441 AGAACTTCCG CCTCAAGTCC TCCCAGTCAC CTAAGGACAT TGACGTGTCC CCCAAACACG 1501 TGGGCTTTGC CACGATCCCA CGAAACTACA CCATGAGCTT CCTGCCCCGC TTGCCAACTT 1561 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1621 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1681 CTTGTGGAAA GGACGAGGTT TCAAGCCTGT GTATCACCGT ACGCGTTAAG TCgacaatca 1741 acctctggat tacaaaattt gtgaaagatt