Construct: ORF TRCN0000469734
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013378.1_s317c1
- Derived from:
- ccsbBroadEn_15309
- DNA Barcode:
- CAGGACATTGCCGCAAGAACTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QRFP (347148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469734
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 347148 | QRFP | pyroglutamylated RFamide pe... | NM_198180.3 | 66.1% | 66.1% | 267_268delGAinsTC;272A>G;274_408del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 366
- ORF length:
- 273
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGTAAG GCCTTACCCC CTGATCTACT 121 TCCTCTTCCT GCCGCTGGGC GCCTGCTTCC CTCTACTGGA CAGAAGAGAG CCCACAGACG 181 CCATGGGTGG CCTCGGAGCT GGAGAACGCT GGGCCGACCT GGCCATGGGG CCCCGACCCC 241 ACTCCGTGTG GGGTTCCTCT CGGTGGCTGA GAGCTTCACA GCCACAGGCC CTGCTTGTCA 301 TAGCCAGGGG GCTGCAGACA TCGGGCAGAG AGCATGCTGG CTGCAGATTC CGCTTCGGTC 361 GGCGGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGGACATT GCCGCAAGAA CTCTAACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt