Construct: ORF TRCN0000469735
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016632.1_s317c1
- Derived from:
- ccsbBroadEn_12812
- DNA Barcode:
- GCCCCATTATATTTTGGCTTGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HVCN1 (84329)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469735
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | NM_001040107.2 | 86.4% | 86.4% | 194_304del |
| 2 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | NM_032369.4 | 86.4% | 86.4% | 194_304del |
| 3 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_005253948.2 | 86.4% | 86.4% | 194_304del |
| 4 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538845.2 | 86.4% | 86.4% | 194_304del |
| 5 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538846.2 | 86.4% | 86.4% | 194_304del |
| 6 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538847.1 | 86.4% | 86.4% | 194_304del |
| 7 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_017020027.1 | 86.4% | 86.4% | 194_304del |
| 8 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_024449225.1 | 86.4% | 86.4% | 194_304del |
| 9 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | NM_001256413.2 | 79.1% | 79.1% | 0_1ins60;134_244del |
| 10 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538838.2 | 73.7% | 73.7% | 20_160del;335_445del |
| 11 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538839.2 | 73.7% | 73.7% | 20_160del;335_445del |
| 12 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538840.3 | 73.7% | 73.7% | 20_160del;335_445del |
| 13 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538841.2 | 73.7% | 73.7% | 20_160del;335_445del |
| 14 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538842.2 | 73.7% | 73.7% | 20_160del;335_445del |
| 15 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_011538844.2 | 73.7% | 73.7% | 20_160del;335_445del |
| 16 | human | 84329 | HVCN1 | hydrogen voltage gated chan... | XM_017020026.2 | 73.7% | 73.7% | 20_160del;335_445del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacctgggac gaaaaggcag tcacccgcag ggccaaggtg gctcccgctg 121 agaggatgag caagttctta aggcacttca cggtcgtggg agacgactac catgcctgga 181 acatcaacta caagaaatgg gagaatgaag aggaggagga ggaggaggag cagccaccac 241 ccacaccagt ctcaggcgag gtcatcatca tctgcttggt ggttctggat gccctcctgg 301 tgcttgctga gctcatcctg gacctgaaga tcatccagcc cgacaagaat aactatgctg 361 ccatggtatt ccactacatg agcatcacca tcttggtctt ttttatgatg gagatcatct 421 ttaaattatt tgtcttccgc ctggagttct ttcaccacaa gtttgagatc ctggatGCCG 481 TCGTGGTGGT GGTCTCATTC ATCCTCGACA TTGTCCTCCT GTTCCAGGAG CACCAGTTTG 541 AGGCTCTGGG CCTGCTGATT CTGCTCCGGC TGTGGCGGGT GGCCCGGATC ATCAATGGGA 601 TTATCATCTC AGTTAAGACA CGTTCAGAAC GGCAACTCTT AAGGTTAAAA CAGATGAATG 661 TACAATTGGC CGCCAAGATT CAACACCTTG AGTTCAGCTG CTCTGAGAAG GAACAAGAAA 721 TTGAAAGACT TAACAAACTA TTGCGACAGC ATGGACTTCT TGGTGAAGTG AACTACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAG CCCCATTATA TTTTGGCTTG TTCACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att