Construct: ORF TRCN0000469742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003210.1_s317c1
- Derived from:
- ccsbBroadEn_01812
- DNA Barcode:
- ATCGTGGCGGGCTTCGCCCCATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF175 (7728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469742
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7728 | ZNF175 | zinc finger protein 175 | NM_007147.4 | 100% | 100% | |
| 2 | human | 7728 | ZNF175 | zinc finger protein 175 | XM_017027249.2 | 92.2% | 92.2% | 0_1ins165 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2199
- ORF length:
- 2133
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgctgatgtg aatttatccc agaagcctca ggtcctgggt ccagagaagc 121 aggatggatc ttgcgaggca tcagtgtcat ttgaggacgt gaccgtggac ttcagcaggg 181 aggagtggca gcaactggac cctgcccaga gatgcctgta ccgggatgtg atgctggagc 241 tctatagcca tctcttcgca gtggggtatc acattcccaa cccagaggtc atcttcagaa 301 tgctaaaaga aaaggagccg cgtgtggagg aggctgaagt ctcacatcag aggtgtcaag 361 aaagggagtt tgggcttgaa atcccacaaa aggagatttc taagaaagct tcatttcaaa 421 aggatatggt aggtgagttc acaagagatg gttcatggtg ttccatttta gaagaactga 481 ggctggatgc tgaccgcaca aagaaagatg agcaaaatca aattcaaccc atgagtcaca 541 gtgctttctt caacaagaaa acattgaaca cagaaagcaa ttgtgaatat aaggaccctg 601 ggaaaatgat tcgcacgagg ccccaccttg cttcttcaca gaaacaacct cagaaatgtt 661 gcttatttac agaaagtttg aagctgaacc tagaagtgaa cggtcagaat gaaagcaatg 721 acacagaaca gcttgatgac gttgttgggt ctggtcagct attcagccat agctcttctg 781 atgcctgcag caagaatatt catacaggag agacattttg caaaggtaac cagtgtagaa 841 aagtctgtgg ccataaacag tcactcaagc aacatcaaat tcatactcag aagaaaccag 901 atggatgttc tgaatgtggg gggagcttca cccagaagtc acacctcttt gcccaacaga 961 gaattcatag tgtaggaaac ctccatgaat gtggcaaatg tggaaaagcc ttcatgccac 1021 aactaaaact cagtgtatat ctgacagatc atacaggtga tataccctgt atatgcaagg 1081 aatgtgggaa ggtctttatt cagagatcag aattgcttac gcaccagaaa acacacacta 1141 gaaagaagcc ctataaatgc catgactgtg gaaaagcctt tttccagatg ttatctctct 1201 tcagacatca gagaactcac agtagagaaa aactctatga atgcagtgaa tgtggcaaag 1261 gcttctccca aaactcaacc ctcattatac atcagaaaat tcatactggt gagagacagt 1321 atgcatgcag tgaatgtggg aaagccttta cccagaagtc aacactcagc ttgcaccaga 1381 gaatccactc agggcagaag tcctatgtgt gtatcgaatg cgggcaggcc ttcatccaga 1441 aggcacacct gattgtccat caaagaagcc acacaggaga aaaaccttat cagtgccaca 1501 actgtgggaa atccttcatt tccaagtcac agcttgatat acatcatcga attcatacag 1561 gggagaaacc ttatgaatgc agtgactgtg gaaaaacctt cacccaaaag tcacacctga 1621 atatacacca gaaaattcat actggagaaa gacaccatgt atgcagtgaa tgcgggaaag 1681 ccttcaacca gaagtcaata ctcagcatgc atcagagaat tcacaccgga gagaagcctt 1741 acaaatgcag tgaatgtggg aaagccttca cttctaagtc tcaattcaaa gagcaTCAGC 1801 GAATTCACAC GGGTGAGAAA CCCTATGTGT GCACTGAATG TGGGAAGGCC TTCAACGGCA 1861 GGTCAAATTT CCATAAACAT CAAATAACTC ACACTAGAGA GAGGCCTTTT GTCTGTTACA 1921 AATGTGGGAA GGCTTTTGTC CAGAAATCAG AGTTGATTAC CCATCAAAGA ACTCACATGG 1981 GAGAGAAACC CTATGAATGC CTTGACTGTG GGAAATCGTT CAGTAAGAAA CCACAACTCA 2041 AGGTGCATCA GCGAATTCAC ACGGGAGAAA GACCTTATGT GTGTTCTGAA TGTGGAAAGG 2101 CCTTCAACAA CAGGTCAAAC TTCAATAAAC ACCAAACAAC TCATACCAGA GACAAATCTT 2161 ACAAATGCAG TTATTCTGTG AAAGGCTTTA CCAAGCAATG CCCAACTTTC TTGTACAAAG 2221 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2281 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2341 ACGAATCGTG GCGGGCTTCG CCCCATGTAC GCGTTAAGTC gacaatcaac ctctggatta 2401 caaaatttgt gaaagatt