Construct: ORF TRCN0000469752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004636.1_s317c1
- Derived from:
- ccsbBroadEn_13477
- DNA Barcode:
- ATTAATGTGCACAAGCTCACAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUN3 (256979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469752
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256979 | SUN3 | Sad1 and UNC84 domain conta... | NM_001284350.2 | 88.3% | 84.8% | 1_22del;49_146del;343A>G |
| 2 | human | 256979 | SUN3 | Sad1 and UNC84 domain conta... | NM_001030019.2 | 85.3% | 85.1% | 1_156del;379A>G |
| 3 | human | 256979 | SUN3 | Sad1 and UNC84 domain conta... | NM_152782.4 | 85.3% | 85.1% | 1_156del;379A>G |
| 4 | human | 256979 | SUN3 | Sad1 and UNC84 domain conta... | XM_017011930.1 | 85.3% | 85.1% | 1_156del;379A>G |
| 5 | human | 256979 | SUN3 | Sad1 and UNC84 domain conta... | XR_002956417.1 | 59.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tacactgact tttcttcttg taggactcct aaatcatcag tggcttaaag 121 aaacagatgt tcctcagaaa tccagacaat tatatgccat aattgcagaa tatggttcaa 181 ggctttataa atatcaggcc agacttcgta tgcctaaaga gcaactggaa cttttaaaga 241 aggaaagcca gaatctggaa aacaattttc gtcaaattct atttttggtc gaacaaatag 301 atgtcctgaa ggcattgcta agagatatga aggatggtat ggacaataat cacaactgga 361 acacccatgg agaccctgtg gaggacccgg accacacaga ggaagtgtca aacttggtca 421 attatgtact taaaaagttg agagaagacc aagtcgagat ggctgattat gccctgaagt 481 cggccggagc ctccatcatt gaagctggga cctcagaaag ttataaaaat aataaagcaa 541 aattgtactg gcatgggata ggtttcCTAA ATCATGAAAT GCCTCCAGAT ATTATTCTTC 601 AGCCGGATGT CTACCCTGGA AAGTGCTGGG CTTTTCCAGG TTCCCAGGGT CATACCCTAA 661 TCAAGCTTGC TACAAAGATC ATACCAACTG CTGTTACCAT GGAGCACATC TCAGAGAAGG 721 TGTCTCCGTC AGGAAACATC TCCAGTGCAC CCAAGGAATT TTCTGTCTAT GGCATCACAA 781 AAAAATGTGA AGGAGAAGAA ATTTTCCTAG GTCAGTTTAT ATATAACAAA ACAGGAACCA 841 CCGTTCAAAC ATTTGAACTC CAGCATGCAG TTTCTGAATA TTTATTATGT GTGAAACTTA 901 ATATCTTTAG CAACTGGGGA CACCCGAAGT ATACTTGTTT ATATCGATTC AGGGTCCATG 961 GCACACCAGG CAAGCACATC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATTA ATGTGCACAA 1141 GCTCACAGGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt