Construct: ORF TRCN0000469778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015384.1_s317c1
- Derived from:
- ccsbBroadEn_00293
- DNA Barcode:
- AGCTTGAGTTCATTGCGGATAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CETN1 (1068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469778
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1068 | CETN1 | centrin 1 | NM_004066.3 | 100% | 100% | |
| 2 | mouse | 26369 | Cetn1 | centrin 1 | NM_007593.5 | 85% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttccggcttc aagaagccca gcgctgcctc caccggccaa aagagaaagg 121 tggcacctaa gcccgagctc actgaggatc agaagcaaga agttcgggaa gcatttgacc 181 TCTTCGACGT GGACGGAAGT GGGACCATCG ACGCGAAGGA GCTGAAGGTG GCCATGAGAG 241 CGCTGGGCTT CGAACCCAGG AAGGAAGAGA TGAAGAAAAT GATCTCCGAG GTGGACAGGG 301 AAGGCACGGG GAAGATCAGC TTCAATGACT TCCTGGCCGT GATGACGCAG AAGATGTCCG 361 AGAAGGACAC CAAAGAAGAA ATCCTGAAGG CCTTCAGGCT CTTTGATGAC GATGAGACCG 421 GGAAGATCTC GTTCAAAAAC CTGAAGCGTG TGGCCAACGA GCTGGGGGAG AACCTCACGG 481 ATGAGGAGCT GCAGGAGATG ATCGACGAAG CTGATCGGGA TGGGGACGGC GAAGTGAACG 541 AGGAGGAGTT CCTTCGGATC ATGAAGAAGA CCAGCCTTTA CTGCCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAAGC TTGAGTTCAT TGCGGATAAA CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t