Construct: ORF TRCN0000469781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007276.1_s317c1
- Derived from:
- ccsbBroadEn_08405
- DNA Barcode:
- ATTATCCAAACTTGCCTTACTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPN2 (54707)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54707 | GPN2 | GPN-loop GTPase 2 | NM_018066.4 | 99.8% | 99.6% | 790A>G |
| 2 | mouse | 100210 | Gpn2 | GPN-loop GTPase 2 | NM_133884.2 | 91% | 96.4% | (many diffs) |
| 3 | mouse | 100210 | Gpn2 | GPN-loop GTPase 2 | NM_001290742.1 | 84.3% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 996
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggggccgct ccgaccacgg ccttcgggca ggcggtgatc ggcccgccgg 121 gctcagggaa gaccacgtac tgcctgggca tgagtgagtt cctgcgcgcg ctgggccggc 181 gcgtggcggt ggtgaacctg gacccggcca acgaggggct gccgtacgag tgtgccgtgg 241 acgtgggcga gctggtgggg ctgggcgacg tgatggacgc gctgcgcctg gggcccaacg 301 gcggcctgct ctactgcatg gagtacctgg aagccaacct ggactggctg cgtgccaagc 361 tcgaccccct ccgcggccac tacttcctct tcgactgccc aggccaggtg gagctctgca 421 cgcatcacgg cgccttgcgc agcatcttct cccaaatggc gcagtgggac ctcaggctga 481 ctgccgtcca cctcgtggat tctcactact gcacagaccc tgccaagttc atttcagtac 541 tgtgtacctc cctggccacc atgctgcacg tggaactgcc ccacatcaac ctcctttcca 601 agatggacct cattgagcat tatgggaagc tggccTTCAA CCTGGACTAC TACACAGAGG 661 TTCTGGACCT CTCCTACCTG CTTGACCACC TGGCTTCTGA CCCTTTCTTC CGCCACTACC 721 GCCAGCTCAA TGAGAAGCTA GTGCAGCTCA TCGAAGACTA TAGCCTTGTC TCCTTTATCC 781 CTCTCAACAT CCAGGACAAG GAGAGCATCC AGCGAGTCCT GCAGGCTGTG GATAAAGCCA 841 ATGGATACTG TTTCGGAGCC CAAGAGCAGC GAAGCTTGGA AGCCATGATG TCTGCCGCAA 901 TGGGAGCCGA CTTCCATTTC TCTTCCACAC TGGGCATCCA GGAGAAGTAC CTGGCACCCT 961 CGAACCAGTC AGTGGAGCAG GAAGCCATGC AGCTGTACCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 AATTATCCAA ACTTGCCTTA CTGTAACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt