Construct: ORF TRCN0000469800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011065.1_s317c1
- Derived from:
- ccsbBroadEn_15616
- DNA Barcode:
- AAGAGAAACTATGATTGGGCAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUFT1 (7286)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7286 | TUFT1 | tuftelin 1 | NM_001126337.2 | 100% | 100% | |
| 2 | human | 7286 | TUFT1 | tuftelin 1 | XM_017002223.2 | 96.3% | 92.5% | (many diffs) |
| 3 | human | 7286 | TUFT1 | tuftelin 1 | XM_017002224.1 | 94.6% | 94.5% | 1_2delATins59 |
| 4 | human | 7286 | TUFT1 | tuftelin 1 | NM_020127.3 | 93.5% | 93.5% | 60_134del |
| 5 | human | 7286 | TUFT1 | tuftelin 1 | XM_011509961.2 | 90.6% | 90.6% | 59_60ins102 |
| 6 | human | 7286 | TUFT1 | tuftelin 1 | NM_001301317.2 | 88% | 84% | (many diffs) |
| 7 | mouse | 22156 | Tuft1 | tuftelin 1 | NM_001293728.1 | 88.8% | 88.4% | (many diffs) |
| 8 | mouse | 22156 | Tuft1 | tuftelin 1 | NM_011656.3 | 83.1% | 82.8% | (many diffs) |
| 9 | mouse | 22156 | Tuft1 | tuftelin 1 | XM_006501307.1 | 82% | 81.6% | (many diffs) |
| 10 | mouse | 22156 | Tuft1 | tuftelin 1 | XM_006501308.2 | 82% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgggacgcgg aactggtgta ccctggtgga cgtgcaccca gaggaccagg 121 cggcggcggg caggaagacc tatgccatgg tgtccagcca ctcagctggt cattctctgg 181 cttcagaact ggtggagtcc catgatggac atgaggagat cattaaggtg tacttgaagg 241 ggaggtctgg agacaagatg attcacgaga agaatattaa ccagctgaag agtgaggtcc 301 agtacatcca ggaggccagg aactgcctac agaagctccg ggaggatata agtagcaagc 361 ttgacaggaa cctaggagat tctctccatc gacaggagat acaggtggtg ctagaaaagc 421 caaatggctt tagtcagagt cccacagccc tgtacagcag cccacctgag gtggacacct 481 gtataaatga ggatgttgag agcttgagga agacggtgca ggacttgctg gccaagcttc 541 aggaggccaa gcggcaacac cagtcagact gtgtggcttt tgaggtcaca ctcagccggt 601 accagaggga agcagaacaa agtaatgtgg cccttcagag agaggaggac agagtggagc 661 agaaagAGGC AGAAGTCGGA GAGCTGCAGA GGCGCTTGCT AGGGATGGAG ACGGAGCATC 721 AGGCCTTACT GGCGAAAGTG AGGGAAGGGG AGGTGGCCCT AGAGGAACTT CGGAGCAACA 781 ATGCTGACTG CCAAGCAGAA CGAGAAAAGG CTGCTACCCT GGAAAAGGAA GTGGCCGGGT 841 TGCGGGAGAA GATCCACCAC TTGGATGACA TGCTCAAGAG CCAGCAGCGG AAAGTCCGGC 901 AAATGATAGA GCAGCTCCAG AATTCAAAAG CTGTGATCCA GTCAAAGGAC GCCACCATCC 961 AGGAGCTCAA GGAGAAAATC GCCTATCTGG AGGCAGAGAA TTTAGAGATG CATGACCGGA 1021 TGGAACACCT GATAGAAAAA CAAATCAGTC ATGGCAACTT CAGCACCCAG GCCCGGGCCA 1081 AGACAGAGAA CCCGGGCAGT ATTAGGATAT CCAAGCCGCC TAGCCCGAAG CCCATGCCTG 1141 TCATCCGAGT GGTGGAAACC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1201 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1261 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAAGA GAAACTATGA 1321 TTGGGCAAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt