Construct: ORF TRCN0000469810
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012208.1_s317c1
- Derived from:
- ccsbBroadEn_15809
- DNA Barcode:
- GTAAGGTAAATATTGCAAGTTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CERS2 (29956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469810
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29956 | CERS2 | ceramide synthase 2 | NM_022075.5 | 99.9% | 100% | 669C>T |
| 2 | human | 29956 | CERS2 | ceramide synthase 2 | NM_181746.3 | 99.9% | 100% | 669C>T |
| 3 | human | 29956 | CERS2 | ceramide synthase 2 | XM_011509452.3 | 99.9% | 100% | 669C>T |
| 4 | human | 29956 | CERS2 | ceramide synthase 2 | XM_011509451.2 | 94.9% | 95% | 1_60del;729C>T |
| 5 | mouse | 76893 | Cers2 | ceramide synthase 2 | NM_001320492.1 | 90.7% | 92.3% | (many diffs) |
| 6 | mouse | 76893 | Cers2 | ceramide synthase 2 | NM_029789.2 | 90.7% | 92.3% | (many diffs) |
| 7 | mouse | 76893 | Cers2 | ceramide synthase 2 | XM_006502260.1 | 90.7% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ccagaccttg tatgattact tctggtggga acgtctgtgg ctgcctgtga 121 acttgacctg ggccgatcta gaagaccgag atggacgtgt ctacgccaaa gcctcagatc 181 tctatatcac gctgcccctg gccttgctct tcctcatcgt tcgatacttc tttgagctgt 241 acgtggctac accactggct gccctcttga acataaagga gaaaactcgg ctgcgggcac 301 ctcccaacgc caccttggaa catttctacc tgaccagtgg caagcagccc aagcaggtgg 361 aagtagagct tttgtcccgg cagagcgggc tctctggccg ccaggtagag cgttggttcc 421 gtcgccgccg caaccaggac cggcccagtc tcctcaagaa gttccgagaa gccagctgga 481 gattcacatt ttacctgatt gccttcattg ccggcatggc cgtcattgtg gataaaccct 541 ggttctatga catgaagaaa gtttgggagg gatatcccat acagagcact atcccttccc 601 agtattggta ctacatgatt gaactttcct tctactggtc cctgctcttc agcattgcct 661 ctgatgtcaa gcgaaaggat ttcaaggaac agatcatcca ccatgtggcc accatcatTC 721 TCATCAGCTT TTCTTGGTTT GCCAATTACA TCCGAGCTGG GACTCTAATC ATGGCTCTGC 781 ATGACTCTTC CGATTACCTG CTGGAGTCAG CCAAGATGTT TAACTACGCG GGATGGAAGA 841 ACACCTGCAA CAACATCTTC ATCGTCTTCG CCATTGTTTT TATCATCACC CGACTGGTCA 901 TCCTGCCCTT CTGGATCCTG CATTGCACCC TGGTGTACCC ACTGGAGCTC TATCCTGCCT 961 TCTTTGGCTA TTACTTCTTC AATTCCATGA TGGGAGTTCT ACAGCTGCTG CATATCTTCT 1021 GGGCCTACCT CATTTTGCGC ATGGCCCACA AGTTCATAAC TGGAAAGCTG GTAGAAGATG 1081 AACGCAGTGA CCGGGAAGAA ACAGAGAGCT CAGAGGGGGA GGAGGCTGCA GCTGGGGGAG 1141 GAGCAAAGAG CCGGCCCCTA GCCAATGGCC ACCCCATCCT CAATAACAAC CATCGTAAGA 1201 ATGACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTAAGGTAA ATATTGCAAG TTACAACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt