Construct: ORF TRCN0000469811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009698.1_s317c1
- Derived from:
- ccsbBroadEn_11800
- DNA Barcode:
- AACCTTTGGGAGCGGCCTATGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOT11 (26027)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26027 | ACOT11 | acyl-CoA thioesterase 11 | NM_147161.4 | 37.1% | 37% | 1_102del;765_1782delinsG |
| 2 | human | 26027 | ACOT11 | acyl-CoA thioesterase 11 | NM_015547.4 | 36.3% | 36.2% | 1_102del;765_1821delinsG |
| 3 | mouse | 329910 | Acot11 | acyl-CoA thioesterase 11 | XM_011240556.2 | 33.2% | 34.1% | (many diffs) |
| 4 | mouse | 329910 | Acot11 | acyl-CoA thioesterase 11 | NM_025590.4 | 32.4% | 33.2% | (many diffs) |
| 5 | mouse | 329910 | Acot11 | acyl-CoA thioesterase 11 | NM_001347159.1 | 31.3% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agacggcgag ggataccgga accccacgga ggtgcagatg agccagctgg 121 tgctgccctg ccacaccaac caacgtggtg agctgagcgt cgggcagctg ctcaagtgga 181 ttgacaccac ggcttgcctg tccgcggaga ggcacgctgg ctgccccTGT GTCACAGCTT 241 CCATGGATGA CATCTATTTT GAGCACACCA TTAGTGTTGG ACAAGTGGTG AATATCAAGG 301 CCAAGGTGAA CCGGGCCTTC AACTCCAGCA TGGAGGTGGG CATCCAGGTG GCCTCGGAGG 361 ACCTGTGCTC TGAGAAGCAG TGGAATGTGT GCAAGGCCTT GGCCACCTTC GTGGCCCGCC 421 GAGAGATCAC CAAGGTGAAG CTGAAGCAGA TCACGCCGCG GACAGAAGAG GAGAAGATGG 481 AGCACAGTGT GGCGGCTGAG CGCCGGCGCA TGCGCCTTGT CTATGCAGAC ACCATCAAGG 541 ACCTCCTGGC CAACTGCGCC ATTCAGGGCG ATCTGGAGAG CAGAGACTGT AGCCGCATGG 601 TGCCGGCTGA GAAGACCCGT GTGGAGAGTG TGGAGCTGGT CCTGCCTCCC CACGCCAATC 661 ACCAGGGCAA CACCTTTGGG GGCCAGATCA TGGCCTGGAT GGAGAATGTG GCCACCATTG 721 CAGCCAGGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACCTT TGGGAGCGGC CTATGTGGAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt