Construct: ORF TRCN0000469823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008934.1_s317c1
- Derived from:
- ccsbBroadEn_07289
- DNA Barcode:
- GCCGTCGTCTTACGACTTTAATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B4GALT4 (8702)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | NM_003778.4 | 99.9% | 99.7% | 346C>G |
| 2 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | NM_212543.2 | 99.9% | 99.7% | 346C>G |
| 3 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_005247855.1 | 99.9% | 99.7% | 346C>G |
| 4 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_006713798.3 | 99.9% | 99.7% | 346C>G |
| 5 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_006713799.3 | 99.9% | 99.7% | 346C>G |
| 6 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_006713800.2 | 99.9% | 99.7% | 346C>G |
| 7 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_006713801.3 | 99.9% | 99.7% | 346C>G |
| 8 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_011513260.1 | 99.9% | 99.7% | 346C>G |
| 9 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_024453804.1 | 99.9% | 99.7% | 346C>G |
| 10 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_024453805.1 | 99.9% | 99.7% | 346C>G |
| 11 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_024453806.1 | 99.9% | 99.7% | 346C>G |
| 12 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_024453807.1 | 99.9% | 99.7% | 346C>G |
| 13 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XM_017007401.1 | 87.9% | 87.7% | 346C>G;671_672ins123 |
| 14 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_001740343.1 | 44.4% | (many diffs) | |
| 15 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_001740340.1 | 43.3% | (many diffs) | |
| 16 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_001740344.2 | 42.8% | (many diffs) | |
| 17 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_001740341.2 | 42% | (many diffs) | |
| 18 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_001740342.2 | 41.8% | (many diffs) | |
| 19 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_001740345.1 | 41.7% | (many diffs) | |
| 20 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_002959604.1 | 40.6% | (many diffs) | |
| 21 | human | 8702 | B4GALT4 | beta-1,4-galactosyltransfer... | XR_001740346.2 | 40.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1098
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cttcaacctg actttccacc tttcctacaa attccgatta ctgttgctgt 121 tgactttgtg cctgacagtg gttgggtggg ccaccagtaa ctacttcgtg ggtgccattc 181 aagagattcc taaagcaaag gagttcatgg ctaatttcca taagaccctc attttgggga 241 agggaaaaac tctgactaat gaagcatcca cgaagaaggt agaacttgac aactgccctt 301 ctgtgtctcc ttacctcaga ggccagagca agctcatttt caaaccagat ctcactttgg 361 aagaggtaca ggcagaaaat cccaaagtgt ccagaggccg gtatcgccct gaggaatgta 421 aagctttaca gagggtcgcc atcctcgttc cccaccggaa cagagagaaa cacctgatgt 481 acctgctgga acatctgcat cccttcctgc agaggcagca gctggattat ggcatctacg 541 tcatccacca ggctgaaggt aaaaagttta atcgagccaa actcttgaat gtgggctatc 601 tagaagccct caaggaagaa aattgggact gctttatatt ccacgatgtg gacctggtac 661 ccgagaatga ctttaaCCTT TACAAGTGTG AGGAGCATCC CAAGCATCTG GTGGTTGGCA 721 GGAACAGCAC TGGGTACAGG TTACGTTACA GTGGATATTT TGGGGGTGTT ACTGCCCTAA 781 GCAGAGAGCA GTTTTTCAAG GTGAATGGAT TCTCTAACAA CTACTGGGGA TGGGGAGGCG 841 AAGACGATGA CCTCAGACTC AGGGTTGAGC TCCAAAGAAT GAAAATTTCC CGGCCCCTGC 901 CTGAAGTGGG TAAATATACA ATGGTCTTCC ACACTAGAGA CAAAGGCAAT GAGGTGAACG 961 CAGAACGGAT GAAGCTCTTA CACCAAGTGT CACGAGTCTG GAGAACAGAT GGGTTGAGTA 1021 GTTGTTCTTA TAAATTAGTA TCTGTGGAAC ACAATCCTTT ATATATCAAC ATCACAGTGG 1081 ATTTCTGGTT TGGTGCATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1201 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCCGTCG TCTTACGACT 1261 TTAATTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt