Construct: ORF TRCN0000469824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017502.1_s317c1
- Derived from:
- ccsbBroadEn_03911
- DNA Barcode:
- AGATTTTTTCAGATCACCTAAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM217B (63939)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63939 | FAM217B | family with sequence simila... | NM_001190826.2 | 100% | 100% | |
| 2 | human | 63939 | FAM217B | family with sequence simila... | NM_022106.3 | 100% | 100% | |
| 3 | human | 63939 | FAM217B | family with sequence simila... | XM_011528985.3 | 96.4% | 96.4% | 1_42del |
| 4 | human | 63939 | FAM217B | family with sequence simila... | XM_011528986.2 | 96.4% | 96.4% | 1_42del |
| 5 | human | 63939 | FAM217B | family with sequence simila... | NM_001190827.1 | 59% | 59% | 0_1ins471 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1215
- ORF length:
- 1149
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgctggccca tcttggaata aagtgcaaca ttcaaagaat tcttcaggaa 121 aaaggcagag taaatcccaa gtaccccacg cttcttccca gccgagaagc agcctcacag 181 ctgtcaccca gcctactgaa gaaaaactta aagaaagcat ttccccggaa gcaagacgca 241 aaaggaatcc actcggttcc aggtgtcagg gggcctcagg gaataaactg tttcttgatt 301 ttcagtcaat gaaaattatt aaagagaatg ctgatgagga cagtgcaagt gatctctctg 361 attcggaaag aattcccatt cctccttctc ccctcacacc tccagatctc aatcttcgag 421 ctgaagaaat tgatccagtt tactttgatc ttcaccctgg tcagggccat acaaaacctg 481 aatactatta tcctaatttc cttccatccc ctttcagctc ctgggaccta cgagatatgg 541 ccctgcttct gaacgcagag aacaaaacgg aagccgtgcc ccgagtggga ggacttcttg 601 ggaagtatat cgatagactt attcagcttg agtggctgca agtccagact gtacagtgtg 661 aaaaagcaaa ggggggcaaa gcaaggcccc ccactgcccc tgggacctca ggggcactga 721 aaagccctgg gagaagtaag ctaattgcta gtgctctgtc CAAGCCACTA CCTCACCAGG 781 AAGGGGCTTC AAAGTCAGGC CCTTCCCGAA AGAAAGCTTT TCACCATGAA GAAATCCACC 841 CATCACATTA TGCATTTGAG ACTTCCCCTA GACCCATTGA TGTGCTTGGT GGTACCAGGT 901 TTTGTTCTCA GAGGCAAACC CTTGAAATGA GGACAGAAGA AAAGAAAAAG AAATCAAGTA 961 AGAGTACGAA GCTGCAGCGC TGGGATCTGT CCGGCAGTGG AAGCAGCTCT AAGGTGGAAA 1021 CCAGCGGTCA CATTCGAGTT CCCAAACAGG CAGCTGTGAT TCTGGACTCA GCAGATTCCT 1081 GTAAGGCCTC CAAAACACAA GCACATGCAC ATCCTAGGAA AAAGGGAAAG GCAGAGAGCT 1141 GTGGTCATGC CACTGTATCG AGTGAGAAAA AACTGAAAAC AAACGGAGTA AAGCAAAACA 1201 CATATAAACT AAAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1261 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1321 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGATTTTTTC AGATCACCTA 1381 AATGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt