Construct: ORF TRCN0000469853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010013.1_s317c1
- Derived from:
- ccsbBroadEn_14122
- DNA Barcode:
- GACACTCCACTGTCACTATATCAA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF21 (27242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27242 | TNFRSF21 | TNF receptor superfamily me... | NM_014452.5 | 72% | 72% | 1_549del |
| 2 | human | 27242 | TNFRSF21 | TNF receptor superfamily me... | XM_017010744.2 | 37.1% | 35.3% | (many diffs) |
| 3 | mouse | 94185 | Tnfrsf21 | tumor necrosis factor recep... | NM_178589.3 | 62.9% | 64.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1482
- ORF length:
- 1416
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa atgcaaagca tacacagact gtctgagtca gaacctggtg gtgatcaagc 121 cggggaccaa ggagacagac aacgtctgtg gcacactccc gtccttctcc agctccacct 181 caccttcccc tggcacagcc atctttccac gccctgagca catggaaacc catgaagtcc 241 cttcctccac ttatgttccc aaaggcatga actcaacaga atccaactct tctgcctctg 301 ttagaccaaa ggtactgagt agcatccagg aagggacagt ccctgacaac acaagctcag 361 caagggggaa ggaagacgtg aacaagaccc tcccaaacct tcaggtagtc aaccaccagc 421 aaggccccca ccacagacac atcctgaagc tgctgccgtc catggaggcc actgggggcg 481 agaagtccag cacgcccatc aagggcccca agaggggaca tcctagacag aacctacaca 541 agcattttga catcaatgag catttgccct ggatgattgt gcttttcctg ctgctggtgc 601 ttgtggtgat tgtggtgtgc agtatccgga aaagctcgag gactctgaaa aaggggcccc 661 ggcaggatcc cagtgccatt gtggaaaagg cagggctgaa gaaatccatg actccaaccc 721 agaaccggga gaaatggatc tactactgca atggccatgg tatcgatatc ctgaagcttg 781 tagcagccca agtgggaagc cagtggaaag atatctatca gtttctttgc aatgccagtg 841 agagggaggt tgctgctttc tccaatgggt acacagccga ccacgagcgg gcctacgcag 901 ctctgcagca ctggaccatc cggggccccg aggccagcct cgcccagcta attagcgccc 961 TGCGCCAGCA CCGGAGAAAC GATGTTGTGG AGAAGATTCG TGGGCTGATG GAAGACACCA 1021 CCCAGCTGGA AACTGACAAA CTAGCTCTCC CGATGAGCCC CAGCCCGCTT AGCCCGAGCC 1081 CCATCCCCAG CCCCAACGCG AAACTTGAGA ATTCCGCTCT CCTGACGGTG GAGCCTTCCC 1141 CACAGGACAA GAACAAGGGC TTCTTCGTGG ATGAGTCGGA GCCCCTTCTC CGCTGTGACT 1201 CTACATCCAG CGGCTCCTCC GCGCTGAGCA GGAACGGTTC CTTTATTACC AAAGAAAAGA 1261 AGGACACAGT GTTGCGGCAG GTACGCCTGG ACCCCTGTGA CTTGCAGCCT ATCTTTGATG 1321 ACATGCTCCA CTTTCTAAAT CCTGAGGAGC TGCGGGTGAT TGAAGAGATT CCCCAGGCTG 1381 AGGACAAACT AGACCGGCTA TTCGAAATTA TTGGAGTCAA GAGCCAGGAA GCCAGCCAGA 1441 CCCTCCTGGA CTCTGTTTAT AGCCATCTTC CTGACCTGCT GTACCAACTT TCTTGTACAA 1501 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 1561 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 1621 GGACGAGACA CTCCACTGTC ACTATATCAA ACGCGTTAAG TCgacaatca acctctggat 1681 tacaaaattt gtgaaagatt