Construct: ORF TRCN0000469865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018803.2_s317c1
- Derived from:
- ccsbBroadEn_15235
- DNA Barcode:
- GTCGACCCGACCCCAGCCTTATAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RSKR (124923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124923 | RSKR | ribosomal protein S6 kinase... | NM_001174103.2 | 66.1% | 7.1% | (many diffs) |
| 2 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532965.3 | 55.8% | 8.6% | (many diffs) |
| 3 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | NM_001287206.1 | 55.6% | 8.6% | (many diffs) |
| 4 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388406.3 | 55.2% | (many diffs) | |
| 5 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532966.3 | 50.8% | 9.2% | (many diffs) |
| 6 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388407.3 | 48.4% | (many diffs) | |
| 7 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532967.3 | 44.7% | 9.2% | (many diffs) |
| 8 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388408.3 | 20.4% | (many diffs) | |
| 9 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_001779956.1 | 15.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 363
- ORF length:
- 294
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagcagta agctgtcggc aggggcagca cacccagcag ggggaacacc 121 acccgggtgg ctgtccctca caagcagggt ggcaacatcc ggggtccctg ggcccgaggc 181 tggaagagcc tctggacagg tttgggaacc atcaggtcag atctggaaga actctgggaa 241 ctacgggggc accactatct gcaccaggaa tccctaaagc cagccccagt actggtagag 301 aagcctctgc cagagtggcc agtgcctcag ttcatcaacc tctttctacc agagtttccc 361 attaggccca ttagggggca gcagcagctg aagattttag gcctcgtggc taaaggctcc 421 tttggaactg tcctcaaggt gctagattgc acccagaaag ctgtatttgc agtgaaggtg 481 gtgcccaagg taaaggtcct acagagggat accgtgaggc agtgcaaaga ggaggttagc 541 atccagcgac agatcaacca tccctttgta cacagcttgg gggacagctg gcagggaaaa 601 CGGCACCTTT TCATTATGTG TAGCTACTGC AGCACAGATC TGTACTCCCT TTGGTCGGCT 661 GTTGGCTGCT TTCCTGAGGC TTCCATCCGT CTCTTTGCTG CCGAGTTGGT GCTGGTACTG 721 TGTTATCTCC ATGACTTGGG CATCATGCAT CGAGATGTGA AGATGGAGAA TATTCTTCTA 781 GATGAACGAG GCCATCTGAA ACTGACAGAC TTTGGTCTGT CCCGCCACGT GCCCCAGGGA 841 GCTCAAGCCT ACACTATCTG TGGCACTCTT CAGTACATGG GTGAGAGAGG TTTGCCAACT 901 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 961 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 1021 TCTTGTGGAA AGGACGAGTC GACCCGACCC CAGCCTTATA GACGCGTTAA GTCgacaatc 1081 aacctctgga ttacaaaatt tgtgaaagat t