Construct: ORF TRCN0000469873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012489.1_s317c1
- Derived from:
- ccsbBroadEn_02199
- DNA Barcode:
- TAAATGCTCGACTCGTTCTGCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MINPP1 (9562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9562 | MINPP1 | multiple inositol-polyphosp... | NM_004897.5 | 100% | 100% | |
| 2 | human | 9562 | MINPP1 | multiple inositol-polyphosp... | XM_006718078.3 | 77.4% | 65.1% | 928_929ins134;1131_1132ins196 |
| 3 | human | 9562 | MINPP1 | multiple inositol-polyphosp... | XM_017016965.2 | 65% | 64% | (many diffs) |
| 4 | human | 9562 | MINPP1 | multiple inositol-polyphosp... | NM_001178117.1 | 64% | 57.1% | 833_834ins232;936_937ins293 |
| 5 | human | 9562 | MINPP1 | multiple inositol-polyphosp... | NM_001178118.1 | 57.8% | 57.2% | (many diffs) |
| 6 | human | 9562 | MINPP1 | multiple inositol-polyphosp... | XM_011540379.3 | 57.8% | 57.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1527
- ORF length:
- 1461
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct acgcgcgccc ggctgcctcc tccggacctc cgtagcgcct gccgcggccc 121 tggctgcggc gctgctctcg tcgcttgcgc gctgctctct tctagagccg agggacccgg 181 tggcctcgtc gctcagcccc tatttcggca ccaagactcg ctacgaggat gtcaaccccg 241 tgctattgtc gggccccgag gctccgtggc gggaccctga gctgctggag gggacctgca 301 ccccggtgca gctggtcgcc ctcattcgcc acggcacccg ctaccccacg gtcaaacaga 361 tccgcaagct gaggcagctg cacgggttgc tgcaggcccg cgggtccagg gatggcgggg 421 ctagtagtac cggcagccgc gacctgggtg cagcgctggc cgactggcct ttgtggtacg 481 cggactggat ggacgggcag ctagtagaga agggacggca ggatatgcga cagctggcgc 541 tgcgtctggc ctcgctcttc ccggcccttt tcagccgtga gaactacggc cgcctgcggc 601 tcatcaccag ttccaagcac cgctgcatgg atagcagcgc cgccttcctg caggggctgt 661 ggcagcacta ccaccctggc ttgccgccgc cggacgtcgc agatatggag tttggacctc 721 caacagttaa tgataaacta atgagatttt ttgatcactg tgagaagttt ttaactgaag 781 tagaaaaaaa tgctacagct ctttatcacg tggaagcctt caaaactgga ccagaaatgc 841 agaacatttt aaaaaaagtt gcagctactt tgcaagtgcc agtaaatgat ttaaatgcag 901 atttaattca agtagccttt ttcacctgtt catttgacct ggcaattaaa ggtgttaaat 961 ctccttggtg tgatgttttt gacatagatg atgcaaaggt attagaatat ttaaatgatc 1021 tgaaacaata ttggaaaaga ggatatgggt atactattaa cagtcgatcc agctgcacct 1081 tgtttcagga tatctttcag cacttggaca aagcagttga acagaaacaa aggtctcagc 1141 caatttcttc tccagtcatc cTCCAGTTTG GTCATGCAGA GACTCTTCTT CCACTGCTTT 1201 CTCTCATGGG CTACTTCAAA GACAAGGAAC CCCTAACAGC GTACAATTAC AAAAAACAAA 1261 TGCATCGGAA GTTCCGAAGT GGTCTCATTG TACCTTATGC CTCGAACCTG ATATTTGTGC 1321 TTTACCACTG TGAAAATGCT AAGACTCCTA AAGAACAATT CCGAGTGCAG ATGTTATTAA 1381 ATGAAAAGGT GTTACCTTTG GCTTACTCAC AAGAAACTGT TTCATTTTAT GAAGATCTGA 1441 AGAACCACTA CAAGGACATC CTTCAGAGTT GTCAAACCAG TGAAGAATGT GAATTAGCAA 1501 GGGCTAACAG TACATCTGAT GAACTATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1561 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1621 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAAATGCT 1681 CGACTCGTTC TGCCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1741 aagatt