Construct: ORF TRCN0000469874
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008525.1_s317c1
- Derived from:
- ccsbBroadEn_01533
- DNA Barcode:
- TATTGCCTCCACTAGAGTTCACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST3GAL2 (6483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469874
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6483 | ST3GAL2 | ST3 beta-galactoside alpha-... | NM_006927.4 | 100% | 100% | |
| 2 | mouse | 20444 | St3gal2 | ST3 beta-galactoside alpha-... | NM_009179.3 | 89.2% | 94.2% | (many diffs) |
| 3 | mouse | 20444 | St3gal2 | ST3 beta-galactoside alpha-... | XM_006530786.2 | 89.2% | 94.2% | (many diffs) |
| 4 | mouse | 20444 | St3gal2 | ST3 beta-galactoside alpha-... | XM_006530787.2 | 89.2% | 94.2% | (many diffs) |
| 5 | mouse | 20444 | St3gal2 | ST3 beta-galactoside alpha-... | XM_006530788.1 | 89.2% | 94.2% | (many diffs) |
| 6 | mouse | 20444 | St3gal2 | ST3 beta-galactoside alpha-... | XM_017312624.1 | 89.2% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtgctccctg cgggtgtggt tcctctccgt ggccttcctg ctggtgttca 121 tcatgtccct gctcttcacc tactcgcacc acagcatggc cacgctcccc tacctggact 181 caggggccct ggatgggacg caccgggtga agctggtgcc cggctatgcc ggcctgcagc 241 gcctcagcaa ggagaggctc tcgggcaaga gctgtgcctg tcgccgctgc atgggcgatg 301 ccggtgcctc cgactggttt gacagccact ttgacggtaa catttccccc gtctggaccc 361 gagagaacat ggatcttcca ccggacgtcc agaggtggtg gatgatgctg cagccccagt 421 tcaagtcaca caacaccaat gaggtgctgg agaagctgtt ccagatagtg cctggcgaga 481 acccctaccg cttccgggac ccccaccagt gccggcgctg tgccgtggtg gggaactcgg 541 gcaacctgcg gggctctggc tatgggcagg acgtggacgg gcacaacttc atcatgagga 601 tgaatcaggc gccaaccgtg ggctttgagc aggatgttgg cagccgaacc acccaccatt 661 tcatgtaccc tgagagtgcc aagaacctgc ccgccaacgt cagcttcgtg ctggtgccct 721 tcaaggtcct ggaccttctg tggatcgcca gcgccttgtc cacggggcag atccgattca 781 cctacgcccc agtgaagtcc ttccttcgag tggataaaga aaaggtccag atctacaacc 841 cagccttctt caagtatatc cacgacaggt ggacagagca tCACGGGCGG TACCCTTCCA 901 CGGGGATGCT GGTGCTTTTC TTTGCCCTGC ATGTGTGTGA TGAGGTGAAC GTGTACGGGT 961 TCGGGGCCGA CAGCCGGGGC AACTGGCACC ACTACTGGGA GAACAACCGG TACGCGGGCG 1021 AGTTCCGGAA GACTGGCGTG CACGACGCGG ACTTCGAGGC CCACATCATC GACATGCTGG 1081 CCAAGGCCAG CAAGATCGAA GTCTACCGGG GCAACTGCCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ATATTGCCTC CACTAGAGTT CACCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt