Construct: ORF TRCN0000469907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016839.1_s317c1
- Derived from:
- ccsbBroadEn_10756
- DNA Barcode:
- TCGATGTTAACGCCTCGAGCACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A10 (1468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469907
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1468 | SLC25A10 | solute carrier family 25 me... | NM_001270888.2 | 99.8% | 100% | 264T>C |
| 2 | human | 1468 | SLC25A10 | solute carrier family 25 me... | NM_012140.5 | 96.8% | 96.9% | 264T>C;627_628ins27 |
| 3 | human | 1468 | SLC25A10 | solute carrier family 25 me... | XM_017024220.1 | 82.3% | 82.4% | 0_1ins129;135T>C;498_499ins27 |
| 4 | human | 1468 | SLC25A10 | solute carrier family 25 me... | NM_001270953.1 | 58.5% | 43.8% | (many diffs) |
| 5 | mouse | 27376 | Slc25a10 | solute carrier family 25 (m... | NM_013770.2 | 83.5% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agccgaggcg cgcgtgtcgc gctggtactt cggggggctg gcctcctgcg 121 gggccgcctg ctgcacgcac ccgctggacc tgctcaaggt gcatctgcag acgcagcagg 181 aggtgaagct gcgcatgacg ggcatggcgc tgcgggtggt gcgtaccgac ggcatcctgg 241 cactctacag cggcctgagc gcctcgctgt gcagacagat gacctactcc ctgactcggt 301 tcgccatcta cgagactgtg cgggaccgcg tggccaaggg cagccagggg cctctcccct 361 tccacgagaa ggtgttgctg ggctccgtca gcggtttagc tggaggcttc gtggggacgc 421 ccgcagactt ggtcaacgtc aggatgcaga acgacgtgaa gctgccccag ggtcagcggc 481 gcaactacgc ccatgcgctg gatggcctgt accgcgtagc tcgtgaagag ggtctcagga 541 gactgttctc gggtgcaacc atggcatcca gccgaggggc cttagtcact gtgggccagc 601 tgtccTGCTA CGACCAGGCC AAGCAGCTGG TCCTTAGCAC CGGGTACCTC TCTGACAACA 661 TCTTCACTCA CTTTGTCGCC AGCTTTATTG CAGCCGCTGG TGACGAGCCC CCTCCTCAGG 721 GTGGATGTGC CACGTTCCTG TGCCAGCCCC TGGATGTGCT GAAGACTCGC CTGATGAACT 781 CCAAGGGGGA GTATCAGGGC GTTTTCCACT GCGCCGTGGA GACAGCGAAG CTCGGGCCTC 841 TGGCCTTTTA CAAGGGCCTC GTCCCAGCTG GCATCCGCCT CATCCCCCAC ACCGTGCTCA 901 CTTTTGTGTT TCTGGAACAG CTACGCAAAA ACTTTGGCAT CAAAGTGCCA TCCTGCCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAT CGATGTTAAC GCCTCGAGCA CTTACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att