Construct: ORF TRCN0000469913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013937.1_s317c1
- Derived from:
- ccsbBroadEn_06980
- DNA Barcode:
- TTTGCAATAAGATCTTTCACCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUMO3 (6612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469913
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6612 | SUMO3 | small ubiquitin like modifi... | NM_006936.3 | 99.6% | 100% | 6C>T |
| 2 | human | 6613 | SUMO2 | small ubiquitin like modifi... | NM_006937.4 | 72.9% | 87.5% | (many diffs) |
| 3 | human | 6612 | SUMO3 | small ubiquitin like modifi... | NM_001286416.1 | 72.8% | 73% | 6C>T;149_262del |
| 4 | human | 6613 | SUMO2 | small ubiquitin like modifi... | NM_001005849.2 | 53.5% | 64.4% | (many diffs) |
| 5 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NM_019929.4 | 80% | 82.4% | (many diffs) |
| 6 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NM_001301673.1 | 77.9% | 80.3% | (many diffs) |
| 7 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NM_001301671.1 | 77.5% | 77.1% | (many diffs) |
| 8 | mouse | 170930 | Sumo2 | small ubiquitin-like modifi... | NM_133354.2 | 72.9% | 87.5% | (many diffs) |
| 9 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NM_001301672.1 | 68.7% | 70.1% | (many diffs) |
| 10 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NR_125904.1 | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgTC TGAGGAGAAG CCCAAGGAGG GTGTGAAGAC AGAGAATGAC CACATCAACC 121 TGAAGGTGGC CGGGCAGGAC GGCTCCGTGG TGCAGTTCAA GATCAAGAGG CACACGCCGC 181 TGAGCAAGCT GATGAAGGCC TACTGCGAGA GGCAGGGCTT GTCAATGAGG CAGATCAGAT 241 TCAGGTTCGA CGGGCAGCCA ATCAATGAAA CTGACACTCC AGCACAGCTG GAGATGGAGG 301 ACGAGGACAC CATCGACGTG TTCCAGCAGC AGACGGGAGG TGTGCCGGAG AGCAGCCTGG 361 CAGGGCACAG TTTCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTTGCAATAA GATCTTTCAC 541 CTAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt