Construct: ORF TRCN0000469914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000868.1_s317c1
- Derived from:
- ccsbBroadEn_12036
- DNA Barcode:
- AGCAAGGGCTTCCCGTTAATATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC10 (54431)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NM_001271581.3 | 10.3% | 10.4% | 1_2007del;2103A>G |
| 2 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NM_018981.4 | 9.7% | 9.8% | 1_2145del;2241A>G |
| 3 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073367.2 | 1.1% | 1_2468del;2564A>G;2703_20037del | |
| 4 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073365.2 | 1.1% | 1_3329del;3425A>G;3564_20898del | |
| 5 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073366.2 | 1.1% | 1_3432del;3528A>G;3667_21001del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 324
- ORF length:
- 234
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATTAAAGG AAAAGTGAAA GCTGGAAAAG 121 TAGACTGTCA GGCTTATGCT CAGACATGCC AGAAAGCTGG GATCAGGGCC TATCCAACTG 181 TTAAGTTTTA TTTCTACGAA AGAGCAAAGA GAAATTTTCA AGAAGAGCAG ATAAATACCA 241 GAGATGCAAA AGCAATCGCT GCCTTAATAA GTGAAAAATT GGAAACTCTC CGAAATCAAG 301 GCAAGAGGAA TAAGGATGAA CTTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 361 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 421 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCAAGGGCTT 481 CCCGTTAATA TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 541 att