Construct: ORF TRCN0000469923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012831.1_s317c1
- Derived from:
- ccsbBroadEn_15392
- DNA Barcode:
- AACTATCACGCTATGTACGCCCTC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP17A1 (1586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469923
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1586 | CYP17A1 | cytochrome P450 family 17 s... | NM_000102.4 | 99.7% | 99.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggagctcgtg gctctcttgc tgcttaccct agcttatttg ttttggccca 121 agagaaggtg ccctggtgcc aagtacccca agagcctcct gtccctgccc ctggtgggca 181 gcctgccatt cctccccaga catggccata tgcataacaa cttcttcaag ctgcagaaaa 241 aatatggccc catctattct gttcgtatgg gcaccaagac tacagtgatt gtcggccacc 301 accagctggc caaggaggtg cttattaaga agggcaagga cttctctggg cggcctcaaa 361 tggcaactct agacatcgcg tccaacaacc gtaagggtat cgccttcgct gactctggcg 421 cacactggca gctgcatcga aggctggcga tggccacctt tgccctgttc aaggatggcg 481 atcagaagct ggagaagatc atttgtcagg aaatcagtac attgtgtgat atgctggcca 541 cccacaacgg acagtccata gacatctcct ttcctgtctt cgtggcggta accaatgtca 601 tctccttgat ctgcttcaat acctcctaca agaatgggga ccctgagttg aatgtcatac 661 agaattacaa tgaaggcatc atagacaacc tgagcaaaga cagcctggtg gacctagtcc 721 cctggttgaa gattttcccc aacaaaaccc tggaaaaatt aaagagccat gttaaaatac 781 gaaatgatct gctgaataaa atacttgaaa attacaagga gaaattccgg agtgactcta 841 tcaccaacat gctggacaca ctgatgcaag ccaagatgaa ctcagataat ggcaatgctg 901 gcccagatca agactcagag ctgctttcag ataaccacat tctcaccacc ataggggaca 961 tctttggggc tggcgtggag accaccacct ctgtggttaa atggaccctg gccttcctgc 1021 tgcacaatcc tcaggtgaag aagaagctct acgaggagat tgaccagaat gtgggtttca 1081 gccgcacacc aactatcagt gaccgtaacc gtctcctcct gctggaggcc accatccgag 1141 aggtgcttcg cctcaggccc gtggccccta tgctcaTCCC CCACAAGGCC AACGTTGACT 1201 CCAGCATCGG TGAGTTTGCT GTGGACAAGG GCACAGAAGT TATCATCAAT CTGTGGGCGC 1261 TGCATCACAA TGAGAAGGAG TGGCACCAGC CGGATCAGTT CATGCCTGAG CGTTTCTTGA 1321 ATCCAGCGGG GACCCAGCTC ATCTCACCGT CAGTAAGCTA TTTGCCCTTC GGAGCAGGAC 1381 CTCGCTCCTG TATAGGTGAG ATCCTGGCCC GCCAGGAGCT CTTCCTCATC ATGGCCTGGC 1441 TGCTGCAGAG GTTCGACCTG GAAGTGCCAG ATGATGGGCA GCTGCCCTCC CTGGAAGGCA 1501 TCCCCAAGGT GGTCTTTCTG ATCGACTCTT TCAAAGTGAA GATCAAGGTG CGCCAGGCCT 1561 GGAGGGAAGC CCAGGCTGAG GGAGCACCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT 1621 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 1681 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACTAT 1741 CACGCTATGT ACGCCCTCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 1801 gaaagatt