Transcript: Human NM_000102.4

Homo sapiens cytochrome P450 family 17 subfamily A member 1 (CYP17A1), mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
CYP17A1 (1586)
Length:
1750
CDS:
53..1579

Additional Resources:

NCBI RefSeq record:
NM_000102.4
NBCI Gene record:
CYP17A1 (1586)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000102.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000064306 GATCGACTCTTTCAAAGTGAA pLKO.1 1507 CDS 100% 4.950 6.930 N CYP17A1 n/a
2 TRCN0000423688 GGACAAGGGCACAGAAGTTAT pLKO_005 1210 CDS 100% 13.200 9.240 N CYP17A1 n/a
3 TRCN0000429719 TACGAAATGATCTGCTGAATA pLKO_005 765 CDS 100% 13.200 9.240 N CYP17A1 n/a
4 TRCN0000433071 TCACCGTCAGTAAGCTATTTG pLKO_005 1331 CDS 100% 13.200 9.240 N CYP17A1 n/a
5 TRCN0000412980 ATGTCATACAGAATTACAATG pLKO_005 639 CDS 100% 10.800 7.560 N CYP17A1 n/a
6 TRCN0000064304 CAGTCCATAGACATCTCCTTT pLKO.1 539 CDS 100% 4.950 3.465 N CYP17A1 n/a
7 TRCN0000064307 CGGCCTCAAATGGCAACTCTA pLKO.1 338 CDS 100% 4.950 3.465 N CYP17A1 n/a
8 TRCN0000064303 CCACCACCTCTGTGGTTAAAT pLKO.1 969 CDS 100% 1.500 1.050 N CYP17A1 n/a
9 TRCN0000064305 CCATATGCATAACAACTTCTT pLKO.1 193 CDS 100% 0.000 0.000 N CYP17A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000102.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00414 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00414 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000474348 CTCCGTGCTCATTAAGAGACCTCA pLX_317 29% 100% 100% V5 n/a
4 ccsbBroadEn_15392 pDONR223 0% 99.8% 100% None 138C>T;195G>T;1398G>A n/a
5 ccsbBroad304_15392 pLX_304 0% 99.8% 100% V5 138C>T;195G>T;1398G>A n/a
6 TRCN0000469923 AACTATCACGCTATGTACGCCCTC pLX_317 27.4% 99.7% 99.6% V5 (not translated due to frame shift) (many diffs) n/a
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