Construct: ORF TRCN0000469927
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007173.1_s317c1
- Derived from:
- ccsbBroadEn_12450
- DNA Barcode:
- TGTAGGTGAACGGGTATCTCTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFIH1 (64135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469927
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64135 | IFIH1 | interferon induced with hel... | NM_022168.4 | 21.2% | 20.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gaatgggtat tccacagacg agaatttccg ctatctcatc tcgtgcttca 121 gggccagggt gaaaatgtac atccaggtgg agcctgtgct ggactacctg acctttctgc 181 ctgcagaggt gaaggagcag attcagagga cagtcgccac ctccgggaac atgcaggcag 241 ttgaactgct gctgagcacc ttggagaagg gagtctggca ccttggttgg actcgggaat 301 tcgtggaggc cctccggaga accggcagcc cTCTGGCCGC CCGCTACATG AACCCTGAGC 361 TCACGGACTT GCCCTCTCCA TCGTTTGAGA ACGCTCATGA TGAATATCTC CAACTGCTGA 421 ACCTCCTTCA GCCCACTCTG GTGGACAAGC TTCTAGTTAG AGACGTCTTG GATAAGTGCA 481 TGGAGGAGGA ACTGTTGACA ATTGAAGACA GAAACCGGAT TGCTGCTGCA GAAAACAATG 541 GAAATGAATC AGGTGTAAGA GAGCTACTAA AAAGGATTGT GCAGAAAGAA AACTGGTTCT 601 CTGCATTTCT GAATGTTCTT CGTCAAACAG GAAACAATGA ACTTGTCCAA GAGTTAACAG 661 GCTCTGATTG CTCAGAAAGC AATGCAGGTA TTTGTAATTT TACTGAGGAA GATTCTTCAA 721 ATTCTGCCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGTAGG TGAACGGGTA TCTCTATTAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt