Construct: ORF TRCN0000469945
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013538.1_s317c1
- Derived from:
- ccsbBroadEn_07714
- DNA Barcode:
- CTAGCTCGCTAATATATAACGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OS9 (10956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469945
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_001017956.2 | 99.9% | 100% | 1056C>T |
| 2 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_001261420.1 | 99.7% | 99.8% | 790_792delGTA;1059C>T |
| 3 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_001017957.2 | 97.4% | 97.5% | 1056C>T;1361_1362ins45 |
| 4 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | XM_006719201.2 | 97.3% | 97.3% | 790_792delGTA;1059C>T;1364_1365ins45 |
| 5 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_001261421.1 | 94.3% | 94.4% | 479_480ins99;691_693delGTA;960C>T |
| 6 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_006812.4 | 91.7% | 91.7% | 1056C>T;1598_1762del |
| 7 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | XM_005268581.2 | 91.5% | 91.6% | 790_792delGTA;1059C>T;1601_1765del |
| 8 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_001261422.1 | 91.4% | 91.5% | 423_424ins156;900C>T |
| 9 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_001017958.2 | 89.4% | 89.5% | 1056C>T;1361_1362ins45;1553_1717del |
| 10 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | XM_006719200.2 | 89.3% | 89.3% | (many diffs) |
| 11 | human | 10956 | OS9 | OS9 endoplasmic reticulum l... | NM_001261423.1 | 87.8% | 87.9% | 160_161ins177;879C>T;1184_1185ins45 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1902
- ORF length:
- 1836
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggaaacg ctgctgtcca gtttgttagg actgctgctt ctgggactcc 121 tgttacccgc aagtctgacc ggcggtgtcg ggagcctgaa cctggaggag ctgagtgaga 181 tgcgttatgg gatcgagatc ctgccgttgc ctgtcatggg agggcagagc caatcttcgg 241 acgtggtgat tgtctcctct aagtacaaac agcgctatga gtgtcgcctg ccagctggag 301 ctattcactt ccagcgtgaa agggaggagg aaacacctgc ttaccaaggg cctgggatcc 361 ctgagttgtt gagcccaatg agagatgctc cctgcttgct gaagacaaag gactggtgga 421 catatgaatt ctgttatgga cgccacatcc agcaatacca catggaagat tcagagatca 481 aaggtgaagt cctctatctc ggctactacc aatcagcctt cgactgggat gatgaaacag 541 ccaaggcctc caagcagcat cgtcttaaac gctaccacag ccagacctat ggcaatgggt 601 ccaagtgcga ccttaatggg aggccccggg aggccgaggt tcggttcctc tgtgacgagg 661 gtgcaggtat ctctggggac tacatcgatc gcgtggacga gcccttgtcc tgctcttatg 721 tgctgaccat tcgcactcct cggctctgcc cccaccctct cctccggccc ccacccagtg 781 ctgcaccgca ggccatcctc tgtcaccctt ccctacagcc tgaggagtac atggcctacg 841 ttcagaggca agccgactca aagcagtatg gagataaaat catagaggag ctgcaagatc 901 taggccccca agtgtggagt gagaccaagt ctggggtggc accccaaaag atggcaggtg 961 cgagcccgac caaggatgac agtaaggact cagatttctg gaagatgctt aatgagccag 1021 aggaccaggc cccaggaggg gaggaggtgc cggctgagga gcaggaccca agccctgagg 1081 cagcagattc agcttctggt gctcccaatg attttcagaa taacgtgcag gtcaaagtca 1141 ttcgaagccc tgcggatttg attcgattca tagaggagct gaaaggtgga acaaaaaagg 1201 ggaagccaaa tataggccaa gagcagcctg tggatgatgc tgcagaagtc cctcagaggg 1261 aaccagagaa ggaaaggggt gatccagaac ggcagagaga gatggaagaa gaggaggatg 1321 aggatgagga tgaggatgaa gatgaggatg aacggcagtt actgggagaa tttgagaagg 1381 aactggaagg gatcctgctt ccgtcagacc gagaccggct ccgttcggag gtgaaggctg 1441 gcatggagcg ggaactggaa aacatcaTCC AGGAGACAGA GAAAGAGCTG GACCCAGATG 1501 GGCTGAAGAA GGAGTCAGAG CGGGATCGGG CAATGCTGGC TCTCACATCC ACTCTCAACA 1561 AACTCATCAA AAGACTGGAG GAAAAACAGA GTCCAGAGCT GGTGAAGAAG CACAAGAAAA 1621 AGAGGGTTGT CCCCAAAAAG CCTCCCCCAT CACCCCAACC TACAGGGAAA ATTGAGATCA 1681 AAATTGTCCG CCCATGGGCT GAAGGGACTG AAGAGGGTGC ACGTTGGCTG ACTGATGAGG 1741 ACACGAGAAA CCTCAAGGAG ATCTTCTTCA ATATCTTGGT GCCGGGAGCT GAAGAGGCCC 1801 AGAAGGAACG CCAGCGGCAG AAAGAGCTGG AGAGCAATTA CCGCCGGGTG TGGGGCTCTC 1861 CAGGTGGGGA GGGCACAGGG GACCTGGACG AATTTGACTT CTGCCCAACT TTCTTGTACA 1921 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1981 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2041 AGGACGACTA GCTCGCTAAT ATATAACGAA AACGCGTTAA GTCgacaatc aacctctgga 2101 ttacaaaatt tgtgaaagat t