Construct: ORF TRCN0000469948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001976.1_s317c1
- Derived from:
- ccsbBroadEn_14355
- DNA Barcode:
- TGCATAAGCTCCCATATTTGTTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BUD23 (114049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NM_017528.5 | 88.8% | 87.9% | 1_93del;834_835insC |
| 2 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NM_001202560.3 | 83.7% | 82.9% | 1_93del;699_749del;885_886insC |
| 3 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XM_006715847.1 | 82.1% | 81.3% | 1_162del;903_904insC |
| 4 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XM_011515779.2 | 77.1% | 62.5% | 1_93del;638_639ins59;744_745ins41 |
| 5 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XM_011515778.1 | 71.3% | 58% | 1_162del;707_708ins59;813_814ins41 |
| 6 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NR_045512.2 | 54.3% | (many diffs) | |
| 7 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NR_037776.3 | 53.6% | 1_318del;1059_1060insC;1069_1397del | |
| 8 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XR_002956407.1 | 14.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgatatccag accaggatgg ctgggcgagc attggagctt ctttatctgc 121 cagagaataa gccctgttac ctgctggata ttggctgtgg cactgggctg agtggaagtt 181 atctgtcaga tgaagggcac tattgggtgg gcctggatat cagccctgcc atgctggatg 241 aggctgtgga ccgagagata gagggagacc tgctgctggg ggatatgggc cagggcatcc 301 cattcaagcc aggcacattt gatggttgca tCAGCATTTC TGCTGTGCAG TGGCTCTGTA 361 ATGCTAACAA GAAGTCTGAA AACCCTGCCA AGCGCCTGTA CTGCTTTTTT GCTTCTCTTT 421 TTTCTGTTCT CGTCCGGGGA TCCCGAGCTG TCCTGCAGCT GTACCCTGAG AACTCAGAGC 481 AGTTGGAGCT GATCACAACC CAGGCCACAA AGGCAGGCTT CTCCGGTGGC ATGGTGGTAG 541 ACTACCCTAA CAGTGCCAAA GCAAAGAAAT TCTACCTCTG CTTGTTTTCT GGGCCTTCGA 601 CCTTTATACC AGAGGGGCTG AGTGAAAATC AGGATGAAGT TGAACCCAGG GAGTCTGTGT 661 TCACCAATGA GAGGTTCCCA TTAAGGATGT CGAGGCGGGG AATGGTGAGG AAGAGTCGGG 721 CATGGGTGCT GGAGAAGAAG GAGCGGCACA GGCGCCAGGG CAGGGAAGTC AGACCTGACA 781 CCCAGTACAC CGGCCGCAAG CGCAAGCCCC CGCTTCTACC CAACTTTCTT GTACAAAGTG 841 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 901 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 961 GATGCATAAG CTCCCATATT TGTTTAACGC GTTAAGTCga caatcaacct ctggattaca 1021 aaatttgtga aagatt