Construct: ORF TRCN0000469971

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002216.1_s317c1
Derived from:
ccsbBroadEn_06898
DNA Barcode:
GTGATTATTTTGCAAATCCTTAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS14 (6208)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469971
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6208 RPS14 ribosomal protein S14 NM_001025070.2 99.7% 100% 150G>A
2 human 6208 RPS14 ribosomal protein S14 NM_001025071.2 99.7% 100% 150G>A
3 human 6208 RPS14 ribosomal protein S14 NM_005617.4 99.7% 100% 150G>A
4 human 644068 RPS14P3 ribosomal protein S14 pseud... NR_077246.1 50.7% (many diffs)
5 mouse 20044 Rps14 ribosomal protein S14 NM_020600.4 91.8% 100% (many diffs)
6 mouse 102634709 LOC102634709 40S ribosomal protein S14 XR_001781586.1 75.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
519
ORF length:
453
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc acctcgaaag ggGAAGGAAA AGAAGGAAGA ACAGGTCATC AGCCTCGGAC
121CTCAGGTGGC TGAAGGAGAG AATGTATTTG GTGTCTGCCA TATCTTTGCA TCCTTCAATG
181ACACTTTTGT CCATGTCACT GATCTTTCTG GCAAAGAAAC CATCTGCCGT GTGACTGGTG
241GGATGAAGGT AAAGGCAGAC CGAGATGAAT CCTCACCATA TGCTGCTATG TTGGCTGCCC
301AGGATGTGGC CCAGAGGTGC AAGGAGCTGG GTATCACCGC CCTACACATC AAACTCCGGG
361CCACAGGAGG AAATAGGACC AAGACCCCTG GACCTGGGGC CCAGTCGGCC CTCAGAGCCC
421TTGCCCGCTC GGGTATGAAG ATCGGGCGGA TTGAGGATGT CACCCCCATC CCCTCTGACA
481GCACTCGCAG GAAGGGGGGT CGCCGTGGTC GCCGTCTGTG CCCAACTTTC TTGTACAAAG
541TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
601GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
661ACGAGTGATT ATTTTGCAAA TCCTTAAAAC GCGTTAAGTC gacaatcaac ctctggatta
721caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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