Construct: ORF TRCN0000469978
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015639.1_s317c1
- Derived from:
- ccsbBroadEn_12960
- DNA Barcode:
- GTCAATACACGCCATTACGTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCED1B (91523)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469978
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91523 | PCED1B | PC-esterase domain containi... | NM_001281429.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 2 | human | 91523 | PCED1B | PC-esterase domain containi... | NM_138371.3 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 3 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_005269224.5 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 4 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_011538978.2 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 5 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020207.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 6 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020208.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 7 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020209.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 8 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020210.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 9 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020211.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 10 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020212.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 11 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020213.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 12 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020214.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 13 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020215.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
| 14 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020216.1 | 63.3% | 45.4% | 1_360del;929_1037del;1296_1297insTAGACGGACC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttcctgcctc tgggacatct ccaggtatgg tccgaactcc tggagaagct 121 acctggagaa cctggagaac ctgttccagt gcctgggcca ggtgctgccc gagtcttgcc 181 tcctggtgtg gaacacggcc atgcctgtgg gcgaggaagt caccgggggt tttcttccgc 241 ccaagctccg gcggcagaag gccaccttcc tgaaaaacga agtggtcaaa gccaacttcc 301 acagcgccac cgaggcacgt aaacataact tcgatgtact ggacttgcat ttccacttcc 361 gccacgcgag ggagaacctg cactgggacg gggtgcactg gaatggacgt gtgcaccgct 421 gcctctccca gctgctgctg gcccacgtgg ccgacgcctg gggtgtggag ctgccccacc 481 gccaccccgt gggcgagtgg atcaagaaga aaaaacctgg cccgagagtc gaagggccgc 541 cccaggccaa cagaaatcac ccggccttac ctctgtcccc acccttacct tcccccacat 601 accgccccct gcttgggttc ccaccccagc gcttactttc cagtcggatc aattctattg 661 ccattcagat gtcccctcat cagcccatgc aggttTCTTC GTCGAAGACA ATTTTATGGT 721 TGGTCCTCAG CTGCCTATGC CCTTCTTCCC CACACCCCGT TATCAGCGGC CTGCCCCAGT 781 GGTACATAGG GGTTTTGGCA GGTATCGTCC CCGTGGCCCC TATACGCCCT GGGGACAGCG 841 GCCTCGACCT TCAAAGAGAA GGGCCCCAGC CAATCCTGAG CCAAGGCCTC AATAGACGGA 901 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAGT CAATACACGC CATTACGTTC ATACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt