Construct: ORF TRCN0000469999
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009306.1_s317c1
- Derived from:
- ccsbBroadEn_04621
- DNA Barcode:
- GGTAGGTATCTCATTCTACCTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPSTI1 (94240)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469999
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94240 | EPSTI1 | epithelial stromal interact... | NM_001002264.4 | 100% | 100% | |
| 2 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_005266596.1 | 97.3% | 97.3% | 654_655ins33 |
| 3 | human | 94240 | EPSTI1 | epithelial stromal interact... | NM_001330543.2 | 77.4% | 77.3% | 950_951insTTC;952A>C;954_955ins273 |
| 4 | human | 94240 | EPSTI1 | epithelial stromal interact... | NM_033255.5 | 74.7% | 74.6% | (many diffs) |
| 5 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_006719896.4 | 70.2% | 67.8% | (many diffs) |
| 6 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_011535312.2 | 69% | 69% | 0_1ins381 |
| 7 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_011535313.1 | 69% | 69% | 0_1ins381 |
| 8 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_017020853.2 | 69% | 69% | 0_1ins381 |
| 9 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_017020854.1 | 69% | 69% | 0_1ins381 |
| 10 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_017020855.2 | 69% | 69% | 0_1ins381 |
| 11 | human | 94240 | EPSTI1 | epithelial stromal interact... | XM_011535314.1 | 54.9% | 53.6% | (many diffs) |
| 12 | human | 94240 | EPSTI1 | epithelial stromal interact... | NM_001331228.2 | 43.8% | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1296
- ORF length:
- 1230
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cacccgcaat agagtggtga actccgggct cggcgcctcc cctgcctccc 121 gcccgacccg ggatccccag gacccttctg ggcggcaagg ggagctgagc cccgtggaag 181 accagagaga gggtttggag gcagccccta agggcccttc gcgggagagc gtcgtgcacg 241 cgggccagag gcgcacaagt gcatacacct tgatagcacc aaatataaac cggagaaatg 301 agatacaaag aattgcggag caggagctgg ccaacctgga gaagtggaag gagcagaaca 361 gagctaaacc ggttcacctg gtgcccagac ggctaggtgg aagccagtca gaaactgaag 421 tcagacagaa acaacaactc cagctgatgc aatctaaata caagcaaaag ctaaaaagag 481 aagaatctgt aagaatcaag aaggaagctg aagaagctga actccaaaaa atgaaggcaa 541 ttcagagaga gaagagcaat aaactggagg agaaaaaaag acttcaagaa aaccttagaa 601 gagaagcatt tagagagcat cagcaataca aaaccgctga gttcttgagc aaactgaaca 661 cagaatcgcc agacagaagt gcctgtcaaa gtgctgtttg tggcccacaa tcctcaacat 721 ggaaacttcc tatcctgcct agggatcaca gctgggccag aagctgggct tacagagatt 781 ctctaaaggc agaagaaaac agaaaattgc aaaagatgaa ggatgaacaa catcaaaaga 841 gtgaattact ggaactgaaa cggcagcagc aagagcaaga aagagccaaa atccaccaga 901 ctgaacacag gagggtaaat aatgcttttc tggaccgact ccaaggcaaa agtcaaccag 961 gtggcctcga gcaaTCTGGA GGCTGTTGGA ATATGAATAG CGGTAACAGC TGGGGTTCTC 1021 TATTAGTTTT TTCGAGGCAC CTAAGGGTAT ATGAGAAAAT ATTGACTCCT ATCTGGCCTT 1081 CATCAACTGA CCTCGAAAAG CCTCATGAGA TGCTTTTTCT TAATGTGATT TTGTTCAGCC 1141 TCACTGTTTT TACCTTAATT TCAACTGCCC ACACACTTGA CCGTGCAGTC AGGAGTGACT 1201 GGCTTCTCCT TGTCCTCATT TATGCATGTT TGGAGGAGCT GATTCCTGAA CTCATATTTA 1261 ATCTCTACTG CCAGGGAAAT GCTACATTAT TTTTCTACCC AACTTTCTTG TACAAAGTGG 1321 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1381 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1441 AGGTAGGTAT CTCATTCTAC CTTTTACGCG TTAAGTCgac aatcaacctc tggattacaa 1501 aatttgtgaa agatt