Construct: ORF TRCN0000470007
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001487.1_s317c1
- Derived from:
- ccsbBroadEn_06981
- DNA Barcode:
- AATTGCTGCTGCCGCCGTGGAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUMO2 (6613)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470007
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6613 | SUMO2 | small ubiquitin like modifi... | NM_006937.4 | 99.6% | 98.9% | 46G>A |
| 2 | human | 387082 | SUMO4 | small ubiquitin like modifi... | NM_001002255.2 | 93.3% | 86.3% | (many diffs) |
| 3 | human | 6613 | SUMO2 | small ubiquitin like modifi... | NM_001005849.2 | 74.3% | 73.6% | 46G>A;149_150ins72 |
| 4 | human | 6612 | SUMO3 | small ubiquitin like modifi... | NM_006936.3 | 73.2% | 86.5% | (many diffs) |
| 5 | human | 6612 | SUMO3 | small ubiquitin like modifi... | NM_001286416.1 | 54.2% | 63.3% | (many diffs) |
| 6 | mouse | 170930 | Sumo2 | small ubiquitin-like modifi... | NM_133354.2 | 95.4% | 98.9% | (many diffs) |
| 7 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NM_019929.4 | 69.3% | 80.1% | (many diffs) |
| 8 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NM_001301673.1 | 67.5% | 78% | (many diffs) |
| 9 | mouse | 20610 | Sumo3 | small ubiquitin-like modifi... | NM_001301671.1 | 66.9% | 76.5% | (many diffs) |
| 10 | mouse | 102636299 | LOC102636299 | small ubiquitin-related mod... | XR_880878.2 | 19.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 351
- ORF length:
- 285
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cGACGAAAAG CCCAAGGAAG GAGTCAAGAC TGAGAACAAC AATCATATTA 121 ATTTGAAGGT GGCGGGGCAG GATGGTTCTG TGGTGCAGTT TAAGATTAAG AGGCATACAC 181 CACTTAGTAA ACTAATGAAA GCCTATTGTG AACGACAGGG ATTGTCAATG AGGCAGATCA 241 GATTCCGATT TGACGGGCAA CCAATCAATG AAACAGACAC ACCTGCACAG TTGGAAATGG 301 AGGATGAAGA TACAATTGAT GTGTTCCAAC AGCAGACGGG AGGTGTCTAC TGCCCAACTT 361 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 421 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 481 CTTGTGGAAA GGACGAAATT GCTGCTGCCG CCGTGGAATT ACGCGTTAAG TCgacaatca 541 acctctggat tacaaaattt gtgaaagatt