Construct: ORF TRCN0000470030
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013942.1_s317c1
- Derived from:
- ccsbBroadEn_05988
- DNA Barcode:
- ACCACGAAGAGCGTGATTTAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEACAM7 (1087)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470030
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1087 | CEACAM7 | CEA cell adhesion molecule 7 | NM_001291485.2 | 99.8% | 100% | 6G>T |
| 2 | human | 1087 | CEACAM7 | CEA cell adhesion molecule 7 | NM_006890.5 | 99.8% | 100% | 6G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 864
- ORF length:
- 795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggttcccct tcagcctgtc catacagagt gtgcattccc tggcaggggc 121 tcctgctcac agcctcgctt ttaaccttct ggaacctgcc aaacagtgcc cagaccaata 181 ttgatgtcgt gccgttcaat gtcgcagaag ggaaggaggt ccttctagta gtccataatg 241 agtcccagaa tctttatggc tacaactggt acaaagggga aagggtgcat gccaactatc 301 gaattatagg atatgtaaaa aatataagtc aagaaaatgc cccagggccc gcacacaacg 361 gTCGAGAGAC AATATACCCC AATGGAACCC TGCTGATCCA GAACGTCACC CACAATGACG 421 CAGGAATCTA TACCCTACAC GTTATAAAAG AAAATCTTGT GAATGAAGAA GTAACCAGAC 481 AATTCTACGT ATTCTCGGAG CCACCCAAGC CCTCCATCAC CAGCAACAAC TTCAATCCGG 541 TGGAGAACAA AGATATTGTG GTTTTAACCT GTCAACCTGA GACTCAGAAC ACAACCTACC 601 TGTGGTGGGT AAACAATCAG AGCCTCCTGG TCAGTCCCAG GCTGCTGCTC TCCACTGACA 661 ACAGGACCCT CGTTCTACTC AGCGCCACAA AGAATGACAT AGGACCCTAT GAATGTGAAA 721 TACAGAACCC AGTGGGTGCC AGCCGCAGTG ACCCAGTCAC CCTGAATGTC CGCTATGAGT 781 CAGTACAAGC AAGTTCACCT GACCTCTCAG CTGGGACCGC TGTCAGCATC ATGATTGGAG 841 TACTGGCTGG GATGGCTCTG ATATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CCACGAAGAG 1021 CGTGATTTAA CCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att