Construct: ORF TRCN0000470039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000870.1_s317c1
- Derived from:
- ccsbBroadEn_15551
- DNA Barcode:
- TCTTAGTTCCCACGTTCCCGAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGER3 (5733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198716.2 | 100% | 100% | |
| 2 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198717.2 | 97.5% | 97.5% | 1077_1078ins27 |
| 3 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_001126044.2 | 94.5% | 90.4% | (many diffs) |
| 4 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198714.2 | 94.5% | 90.4% | (many diffs) |
| 5 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198719.2 | 94.5% | 90.4% | (many diffs) |
| 6 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198715.3 | 94.2% | 90% | (many diffs) |
| 7 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | XM_011541810.3 | 90.7% | 86.1% | (many diffs) |
| 8 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198718.2 | 89.1% | 86.7% | (many diffs) |
| 9 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | XR_946714.2 | 74.7% | 1_231del;1336_1428del;1447_1501del | |
| 10 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028293.2 | 56.2% | (many diffs) | |
| 11 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028292.2 | 55.5% | (many diffs) | |
| 12 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028294.2 | 53.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggagacccgg ggctacggag gggatgcccc cttctgcacc cgcctcaacc 121 actcctacac aggcatgtgg gcgcccgagc gttccgccga ggcgcggggc aacctcacgc 181 gccctccagg gtctggcgag gattgcggat cggtgtccgt ggccttcccg atcaccatgc 241 tgctcactgg tttcgtgggc aacgcactgg ccatgctgct cgtgtcgcgc agctaccggc 301 gccgggagag caagcgcaag aagtccttcc tgctgtgcat cggctggctg gcgctcaccg 361 acctggtcgg gcagcttctc accaccccgg tcgtcatcgt cgtgtacctg tccaagcagc 421 gttgggagca catcgacccg tcggggcggc tctgcacctt tttcgggctg accatgactg 481 ttttcgggct ctcctcgttg ttcatcgcca gcgccatggc cgtcgagcgg gcgctggcca 541 tcagggcgcc gcactggtat gcgagccaca tgaagacgcg tgccacccgc gctgtgctgc 601 tcggcgtgtg gctggccgtg ctcgccttcg ccctgctgcc ggtgctgggc gtgggccagt 661 acaccgtcca gtggcccggg acgtggtgct tcatcagcac cgggcgaggg ggcaacggga 721 ctagctcttc gcataactgg ggcaaccttt tcttcgcctc tgcctttgcc ttcctggggc 781 tcttggcgct gacagtcacc tttTCCTGCA ACCTGGCCAC CATTAAGGCC CTGGTGTCCC 841 GCTGCCGGGC CAAGGCCACG GCATCTCAGT CCAGTGCCCA GTGGGGCCGC ATCACGACCG 901 AGACGGCCAT TCAGCTTATG GGGATCATGT GCGTGCTGTC GGTCTGCTGG TCTCCGCTCC 961 TGATAATGAT GTTGAAAATG ATCTTCAATC AGACATCAGT TGAGCACTGC AAGACACACA 1021 CGGAGAAGCA GAAAGAATGC AACTTCTTCT TAATAGCTGT TCGCCTGGCT TCACTGAACC 1081 AGATCTTGGA TCCTTGGGTT TACCTGCTGT TAAGAAAGAT CCTTCTTCGA AAGTTTTGCC 1141 AGATGAGAAA AAGAAGACTC AGAGAGCAAG AGGAATTTTG GGGAAATTAC CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGATCTTAGT TCCCACGTTC CCGAAGCACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt