Construct: ORF TRCN0000470073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012987.1_s317c1
- Derived from:
- ccsbBroadEn_02319
- DNA Barcode:
- CTTGCCACCAGACGTTGAATCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PREB (10113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10113 | PREB | prolactin regulatory elemen... | NM_013388.6 | 100% | 100% | |
| 2 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330485.1 | 86% | 86% | 749_750ins174 |
| 3 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330486.1 | 83.9% | 79.3% | 999_1000ins160;1050_1051ins41 |
| 4 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330484.1 | 82.7% | 82.7% | 0_1ins216 |
| 5 | human | 10113 | PREB | prolactin regulatory elemen... | XR_939649.3 | 72.4% | (many diffs) | |
| 6 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330487.1 | 70% | 65.5% | 749_750ins174;825_826ins160;876_877ins41 |
| 7 | human | 10113 | PREB | prolactin regulatory elemen... | NR_138479.1 | 55.8% | 1_254del;1179_1204del;1532_2238del | |
| 8 | mouse | 50907 | Preb | prolactin regulatory elemen... | NM_016703.3 | 86.8% | 88.7% | (many diffs) |
| 9 | mouse | 50907 | Preb | prolactin regulatory elemen... | NM_001294302.1 | 72.7% | 71.4% | (many diffs) |
| 10 | mouse | 50907 | Preb | prolactin regulatory elemen... | XM_006504015.2 | 67% | 65.8% | (many diffs) |
| 11 | mouse | 50907 | Preb | prolactin regulatory elemen... | XM_006504017.3 | 63.5% | 64.5% | (many diffs) |
| 12 | mouse | 50907 | Preb | prolactin regulatory elemen... | XR_376814.3 | 18.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1317
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccggcgccgg gcgccagagc tgtaccgggc tccgttcccg ttgtacgcgc 121 ttcaggtcga ccccagcact gggctgctca tcgctgcggg cggaggaggc gccgccaaga 181 caggcataaa gaatggcgtg cactttctgc agctagagct gattaatggg cgcttgagtg 241 cctccttgct gcactcccat gacacagaga cacgggccac catgaacttg gcactggctg 301 gtgacatcct tgctgcaggg caggatgccc actgtcagct cctgcgcttc caggcacatc 361 aacagcaggg caacaaggca gagaaggccg gttccaagga gcaggggcct cgacaaagga 421 agggagcagc cccagcagag aagaaatgtg gagcggaaac ccagcacgag gggctagaac 481 tcagggtaga gaatttgcag gcggtgcaga cagactttag ctccgatcca ctgcagaaag 541 ttgtgtgctt caaccacgat aataccctgc ttgccactgg aggaacagat ggctacgtcc 601 gtgtctggaa ggtgcccagc ctggagaagg ttctggagtt caaagcccac gaaggggaga 661 ttgaagacct ggctttaggg cctgatggca agttggtaac cgtgggccgg gaccttaagg 721 cctctgtgtg gcagaaggat cagctggtga cacagctgca ctggcaagaa aatggaccca 781 ccttttccag cacaccttac cgctaccagg cctgcaggtt tgggcaggtt ccagaccagc 841 ctgctggcct gcgactcttc acagtgcaaa ttccccacaa gcgcctgcgc cagccccctc 901 ccTGCTACCT CACAGCCTGG GATGGCTCCA ACTTCTTGCC CCTTCGGACC AAGTCCTGTG 961 GCCATGAAGT CGTCTCCTGC CTCGATGTCA GTGAATCCGG CACCTTCCTA GGCCTGGGCA 1021 CAGTCACTGG CTCTGTTGCC ATCTACATAG CTTTCTCTCT CCAGTGCCTC TACTACGTGA 1081 GGGAGGCCCA TGGCATTGTG GTGACGGATG TGGCCTTTCT ACCTGAGAAG GGTCGTGGTC 1141 CAGAGCTCCT TGGGTCCCAT GAAACTGCCC TGTTCTCTGT GGCTGTGGAC AGTCGTTGCC 1201 AGCTGCATCT GTTGCCCTCA CGGCGGAGTG TTCCTGTGTG GCTCCTGCTC CTGCTGTGTG 1261 TCGGGCTTAT TATTGTGACC ATCCTGCTGC TCCAGAGTGC CTTTCCAGGT TTCCTTTACC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GACTTGCCAC CAGACGTTGA ATCATCACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt