Construct: ORF TRCN0000470079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008660.1_s317c1
- Derived from:
- ccsbBroadEn_12239
- DNA Barcode:
- TTGGAGCGAACGATTCTTCTTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYBU (55638)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470079
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55638 | SYBU | syntabulin | XM_011517154.2 | 92.1% | 92.1% | 1_117del |
| 2 | human | 55638 | SYBU | syntabulin | NM_001363032.2 | 91.4% | 91.4% | 1_129del |
| 3 | human | 55638 | SYBU | syntabulin | NM_001330596.2 | 85.7% | 85.7% | 1_228del |
| 4 | human | 55638 | SYBU | syntabulin | NM_001099746.1 | 84% | 84% | 1_261del |
| 5 | human | 55638 | SYBU | syntabulin | NM_001099749.2 | 84% | 84% | 1_261del |
| 6 | human | 55638 | SYBU | syntabulin | NM_001099755.2 | 84% | 84% | 1_261del |
| 7 | human | 55638 | SYBU | syntabulin | XM_017013614.1 | 84% | 84% | 1_261del |
| 8 | human | 55638 | SYBU | syntabulin | XM_017013615.1 | 84% | 84% | 1_261del |
| 9 | human | 55638 | SYBU | syntabulin | NM_001099756.1 | 69.2% | 69.2% | 1_609del |
| 10 | human | 55638 | SYBU | syntabulin | NM_001099743.2 | 69% | 69% | 1_615del |
| 11 | human | 55638 | SYBU | syntabulin | NM_001099747.2 | 69% | 69% | 1_615del |
| 12 | human | 55638 | SYBU | syntabulin | NM_001099751.1 | 69% | 69% | 1_615del |
| 13 | human | 55638 | SYBU | syntabulin | NM_001099753.2 | 69% | 69% | 1_615del |
| 14 | human | 55638 | SYBU | syntabulin | NM_017786.6 | 69% | 69% | 1_615del |
| 15 | human | 55638 | SYBU | syntabulin | NM_001099744.1 | 68.9% | 68.9% | 1_618del |
| 16 | human | 55638 | SYBU | syntabulin | NM_001099745.2 | 68.9% | 68.9% | 1_618del |
| 17 | human | 55638 | SYBU | syntabulin | NM_001099748.1 | 68.9% | 68.9% | 1_618del |
| 18 | human | 55638 | SYBU | syntabulin | NM_001099750.1 | 68.9% | 68.9% | 1_618del |
| 19 | human | 55638 | SYBU | syntabulin | NM_001099752.1 | 68.9% | 68.9% | 1_618del |
| 20 | human | 55638 | SYBU | syntabulin | NM_001099754.2 | 68.9% | 68.9% | 1_618del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1437
- ORF length:
- 1371
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtgcaggaat cagctgagcc ctgtcaatat ccatcccagt tatgcacctt 121 cttccccaag cagtagcaac tcaggctcct acaaaggaag cgactgtagc cccatcatga 181 ggcgttctgg aaggtacatg tcttgcggtg aaaatcatgg tgtcagaccc ccaaacccag 241 agcagtattt gactccactg cagcagaaag aggtgacagt gagacacctc aaaaccaagc 301 tgaaggaatc tgagcgccga ctccatgaaa gggaaagtga aatcgtggag cttaagtccc 361 agctggcccg catgcgagag gactggattg aggaggagtg tcaccgggta gaggcccagt 421 tggcactcaa agaagccagg aaagagatta aacagctcaa acaggtcatc gaaaccatgc 481 ggagcagctt ggctgataaa gataaaggca ttcagaaata ttttgtggac ataaacatcc 541 aaaacaagaa gctggagtct ctccttcaga gcatggagat ggcacacagt ggctctctga 601 gggacgaact gtgcctagac tttccatgtg attccccaga gaagagctta accctcaacc 661 cccctcttga cacaatggca gatgggttat ctctggaaga gcaggtcacg ggggaagggg 721 ctgacaggga gctactggta ggagatagca tagccaacag cacagatttg ttcgatgaga 781 tagtgacagc caccaccaca gaatctggtg acctggagct tgtgcattcc acccctgggg 841 ctaacgtcct ggagctgctg cccatagtca tgggtcagga ggagggcagt gtggtggtgg 901 agcgagccgt tcagaccgac gtggtgccct acagcccagc catctcagag ctcattcaga 961 gtgtgctgca gaagctccag gacccctgTC CCTCGAGCTT GGCGTCCCCT GATGAGTCTG 1021 AACCAGACTC GATGGAGAGC TTCCCAGAGT CCCTCTCTGC CTTAGTGGTT GATTTAACTC 1081 CAAGAAATCC AAACTCAGCC ATCCTTTTGT CTCCCGTGGA GACCCCCTAC GCCAATGTGG 1141 ATGCAGAAGT TCATGCAAAC CGCCTCATGA GAGAGCTGGA TTTTGCAGCC TGCGTGGAAG 1201 AGAGGTTGGA TGGTGTCATC CCACTGGCTC GCGGGGGCGT CGTGAGGCAG TACTGGAGCA 1261 GCAGCTTCCT GGTGGATCTC CTGGCTGTGG CTGCCCCCGT GGTCCCCACG GTTCTGTGGG 1321 CATTCAGTAC TCAGAGAGGG GGAACGGATC CTGTGTATAA CATCGGGGCC TTGCTCAGGG 1381 GCTGTTGCGT GGTTGCCCTG CATTCGCTCC GCCGCACCGC CTTCCGTATC AAAACCTACC 1441 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1501 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1561 ATATATCTTG TGGAAAGGAC GATTGGAGCG AACGATTCTT CTTGTTACGC GTTAAGTCga 1621 caatcaacct ctggattaca aaatttgtga aagatt