Construct: ORF TRCN0000470099

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003823.3_s317c1
Derived from:
ccsbBroadEn_11186
DNA Barcode:
GTCCATCCAGGGGTTAGCCTACAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGFBR2 (7048)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470099
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7048 TGFBR2 transforming growth factor ... NM_003242.6 58.9% 58.9% 1003_1701del
2 human 7048 TGFBR2 transforming growth factor ... NM_001024847.2 56.4% 56.2% 95_169del;1078_1776del
3 human 7048 TGFBR2 transforming growth factor ... XM_011534043.2 54.7% 51.8% (many diffs)
4 human 7048 TGFBR2 transforming growth factor ... XM_011534045.3 52.7% 52.7% 0_1ins105;898_1596del
5 human 7048 TGFBR2 transforming growth factor ... XM_017007106.1 52.7% 52.7% 0_1ins105;898_1596del
6 mouse 21813 Tgfbr2 transforming growth factor,... NM_029575.3 50.4% 52% (many diffs)
7 mouse 21813 Tgfbr2 transforming growth factor,... NM_009371.3 48.7% 49.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1068
ORF length:
1002
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg tcgggggctg ctcaggggcc tgtggccgct gcacatcgtc ctgtggacgc
121gtatcgccag cacgatccca ccgcacgttc agaagtcggt taataacgac atgatagtca
181ctgacaacaa cggtgcagtc aagtttccac aactgtgtaa attttgtgat gtgagatttt
241ccacctgtga caaccagaaa tcctgcatga gcaactgcag catcacctcc atctgtgaga
301agccacagga agtctgtgtg gctgtatgga gaaagaatga cgagaacata acactagaga
361cagtttgcca tgaccccaag ctcccctacc atgactttat tctggaagat gctgcttctc
421caaagtgcat tatgaaggaa aaaaaaaagc ctggtgagac tttcttcatg tgttcctgta
481gctctgatga gtgcaatgac aacatcatct tctcagaaga atataacacc agcaatcctg
541acttgttgct agtcatattt caagtgacag gcatcagcct cctgccacca ctgggagttg
601ccatatctgt catcatcaTC TTCTACTGCT ACCGCGTTAA CCGGCAGCAG AAGCTGAGTT
661CAACCTGGGA AACCGGCAAG ACGCGGAAGC TCATGGAGTT CAGCGAGCAC TGTGCCATCA
721TCCTGGAAGA TGACCGCTCT GACATCAGCT CCACGTGTGC CAACAACATC AACCACAACA
781CAGAGCTGCT GCCCATTGAG CTGGACACCC TGGTGGGGAA AGGTCGCTTT GCTGAGGTCT
841ATAAGGCCAA GCTGAAGCAG AACACTTCAG AGCAGTTTGA GACAGTGGCA GTCAAGATCT
901TTCCCTATGA GGAGTATGCC TCTTGGAAGA CAGAGAAGGA CATCTTCTCA GACATCAATC
961TGAAGCATGA GAACATACTC CAGTTCCTGA CGGCTGAGGA GCGGAAGACG GAGTTGGGGA
1021AACAATACTG GCTGATCACC GCCTTCCACG CCAAGGGCAA CCTACAGTAC CCAACTTTCT
1081TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1141CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1201GTGGAAAGGA CGAGTCCATC CAGGGGTTAG CCTACATACG CGTTAAGTCg acaatcaacc
1261tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)