Construct: ORF TRCN0000470099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003823.3_s317c1
- Derived from:
- ccsbBroadEn_11186
- DNA Barcode:
- GTCCATCCAGGGGTTAGCCTACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGFBR2 (7048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470099
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7048 | TGFBR2 | transforming growth factor ... | NM_003242.6 | 58.9% | 58.9% | 1003_1701del |
| 2 | human | 7048 | TGFBR2 | transforming growth factor ... | NM_001024847.2 | 56.4% | 56.2% | 95_169del;1078_1776del |
| 3 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_011534043.2 | 54.7% | 51.8% | (many diffs) |
| 4 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_011534045.3 | 52.7% | 52.7% | 0_1ins105;898_1596del |
| 5 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_017007106.1 | 52.7% | 52.7% | 0_1ins105;898_1596del |
| 6 | mouse | 21813 | Tgfbr2 | transforming growth factor,... | NM_029575.3 | 50.4% | 52% | (many diffs) |
| 7 | mouse | 21813 | Tgfbr2 | transforming growth factor,... | NM_009371.3 | 48.7% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tcgggggctg ctcaggggcc tgtggccgct gcacatcgtc ctgtggacgc 121 gtatcgccag cacgatccca ccgcacgttc agaagtcggt taataacgac atgatagtca 181 ctgacaacaa cggtgcagtc aagtttccac aactgtgtaa attttgtgat gtgagatttt 241 ccacctgtga caaccagaaa tcctgcatga gcaactgcag catcacctcc atctgtgaga 301 agccacagga agtctgtgtg gctgtatgga gaaagaatga cgagaacata acactagaga 361 cagtttgcca tgaccccaag ctcccctacc atgactttat tctggaagat gctgcttctc 421 caaagtgcat tatgaaggaa aaaaaaaagc ctggtgagac tttcttcatg tgttcctgta 481 gctctgatga gtgcaatgac aacatcatct tctcagaaga atataacacc agcaatcctg 541 acttgttgct agtcatattt caagtgacag gcatcagcct cctgccacca ctgggagttg 601 ccatatctgt catcatcaTC TTCTACTGCT ACCGCGTTAA CCGGCAGCAG AAGCTGAGTT 661 CAACCTGGGA AACCGGCAAG ACGCGGAAGC TCATGGAGTT CAGCGAGCAC TGTGCCATCA 721 TCCTGGAAGA TGACCGCTCT GACATCAGCT CCACGTGTGC CAACAACATC AACCACAACA 781 CAGAGCTGCT GCCCATTGAG CTGGACACCC TGGTGGGGAA AGGTCGCTTT GCTGAGGTCT 841 ATAAGGCCAA GCTGAAGCAG AACACTTCAG AGCAGTTTGA GACAGTGGCA GTCAAGATCT 901 TTCCCTATGA GGAGTATGCC TCTTGGAAGA CAGAGAAGGA CATCTTCTCA GACATCAATC 961 TGAAGCATGA GAACATACTC CAGTTCCTGA CGGCTGAGGA GCGGAAGACG GAGTTGGGGA 1021 AACAATACTG GCTGATCACC GCCTTCCACG CCAAGGGCAA CCTACAGTAC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGAGTCCATC CAGGGGTTAG CCTACATACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt