Construct: ORF TRCN0000470112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010319.1_s317c1
- Derived from:
- ccsbBroadEn_11403
- DNA Barcode:
- TACAACCCATGAGAAGCATTTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM53B (9679)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9679 | FAM53B | family with sequence simila... | NM_014661.4 | 72.1% | 71.5% | 908_924delinsT;929T>C;932_1266del |
| 2 | mouse | 77938 | Fam53b | family with sequence simila... | NM_175268.4 | 62.6% | 63.8% | (many diffs) |
| 3 | mouse | 77938 | Fam53b | family with sequence simila... | NM_212473.1 | 62.6% | 63.8% | (many diffs) |
| 4 | mouse | 77938 | Fam53b | family with sequence simila... | NM_001347630.1 | 57% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gatggtccta agtgaaagcc tcagcacccg gggagctgac tccattgcat 121 gtgggacctt cagccgtgaa ctgcacacgc caaagaagat gagtcaagga cctacacttt 181 tctcttgtgg aattatggaa aatgacagat ggcgagacct ggacaggaaa tgccctcttc 241 agattgacca accgagcacc agcatctggg aatgcctgcc tgaaaaggac agctcactat 301 ggcaccggga ggcagtgacc gcctgcgctg tgaccagtct gatcaaagac ctcagcatca 361 gcgaccacaa cgggaacccc tcagcacccc ctagcaagcg ccagtgccgc tcactgtcct 421 tctccgatga gatgtccagt tgccggacat catggaggcc cttgggctcc aaagtctgga 481 ctcccgtgga aaagagacgc tgctacagcg ggggcagcgt ccagcgctat tccaacggct 541 tcagcaccat gcagaggagt tccagcttca gcctcccttc ccgggccaac gtgctctcct 601 cacccTGCGA CCAGGCAGGA CTCCACCACC GATTTGGAGG GCAGCCCTGC CAAGGGGTGC 661 CAGGCTCAGC CCCGTGTGGA CAGGCAGGTG ACACCTGGAG CCCTGACCTG CACCCCGTGG 721 GAGGAGGCCG GCTGGACCTG CAGCGGTCCC TCTCTTGCTC ACATGAGCAG TTTTCCTTTG 781 TGGAATACTG TCCTCCCTCA GCCAACAGCA CACCTGCCTC AACACCAGAG CTGGCGAGAC 841 GCTCCAGCGG CCTTTCCCGC AGCCGCTCCC AGCCGTGTGT CCTTAACGAC AAGAAGGTCG 901 GTGTTAAAAG GCGGCGCCCT GAAGAAGTGC AAGAGCAGAG GCCTTCTCTA GACCTTGCCA 961 AGATGGCACA GATAGCCCCA AGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACA ACCCATGAGA 1141 AGCATTTATG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt