Construct: ORF TRCN0000470113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011830.1_s317c1
- Derived from:
- ccsbBroadEn_07631
- DNA Barcode:
- TTGAAAACTTTCTATAACAAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BATF (10538)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470113
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10538 | BATF | basic leucine zipper ATF-li... | NM_006399.5 | 99.7% | 99.2% | 365G>C |
| 2 | mouse | 53314 | Batf | basic leucine zipper transc... | NM_016767.2 | 89.8% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgacatgcc TCACAGCTCC GACAGCAGTG ACTCCAGCTT CAGCCGCTCT CCTCCCCCTG 121 GCAAACAGGA CTCATCTGAT GATGTGAGAA GAGTTCAGAG GAGGGAGAAA AATCGTATTG 181 CCGCCCAGAA GAGCCGACAG AGGCAGACAC AGAAGGCCGA CACCCTGCAC CTGGAGAGCG 241 AAGACCTGGA GAAACAGAAC GCGGCTCTAC GCAAGGAGAT CAAGCAGCTC ACAGAGGAAC 301 TGAAGTACTT CACGTCGGTG CTGAACAGCC ACGAGCCCCT GTGCTCGGTG CTGGCCGCCA 361 GCACGCCCTC GCCCCCCGAG GTGGTGTACA GCGCCCACGC ATTCCACCAA CCTCATGTCA 421 GCTCCCCGCC CTTCCAGCCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTGA AAACTTTCTA 601 TAACAAAATA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt