Construct: ORF TRCN0000470121
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003884.1_s317c1
- Derived from:
- ccsbBroadEn_05071
- DNA Barcode:
- AATATGATCATTAAAACATAGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MBLAC2 (153364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470121
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153364 | MBLAC2 | metallo-beta-lactamase doma... | NM_203406.2 | 99.8% | 99.6% | 382A>G |
| 2 | mouse | 72852 | Mblac2 | metallo-beta-lactamase doma... | NM_028372.1 | 91.7% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggcgctcgag tggtacgccc acaagtctct aggcgatggt atcttctgga 121 ttcaagaacg tttctacgag tcgggcaacc gtgccaacat ctggctggtg cgcggctccg 181 agcaggacgt ggtgatcgat acaggcctgg ggctgcgcag cctcccggag tacctgtact 241 cctccggcct cttgcaggac cgagaggcca aagaggacgc ggcgcgccgg ccactgcttg 301 ccgtggccac ccacgtgcac ttcgaccact ccggcggcct ctaccagttc gaccgcgtgg 361 cagtgcacca cgccGAGGCC GAGGCGCTGG CTCGCGGGGA CAACTTTGAG ACCGTGACCT 421 GGCTTTCCGA TAGCGAGGTG GTGCGGGCGC CCAGCCCCGG CTGGAGGGCC AGACAGTTCC 481 GGGTACAGGC GGTGCAGCCC ACCCTCATCC TGCAGGATGG GGATGTGATC AACCTTGGTG 541 ACAGACAGCT CACTGTTATG CACATGCCAG GTCACTCCAG GGGCAGTATT TGCTTACATG 601 ACAAAGACCG AAAGATTCTC TTCAGTGGAG ACGTCGTGTA TGATGGATCA CTGATTGACT 661 GGCTCCCATA CAGCAGGATA AGTGACTATG TTGGAACTTG TGAACGTCTA ATAGAATTAG 721 TGGACAGAGG TCTGGTAGAG AAGGTGCTTC CTGGGCACTT CAATACCTTT GGTGCTGAAA 781 GGCTTTTTCG ATTGGCTTCT AACTATATTT CAAAAGCTGG GATATGTCAC AAAGTTTCTA 841 CTTTTGCCAT GCGATCTCTT GCAAGTTTAG CTCTACGTGT AACAAATTCT AGGACCTCGC 901 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TATGATCATT AAAACATAGT CTACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt