Construct: ORF TRCN0000470144
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012150.1_s317c1
- Derived from:
- ccsbBroadEn_00052
- DNA Barcode:
- ACAACCCTTCAACAGTCACCCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH3A2 (224)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470144
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_001031806.2 | 100% | 100% | |
| 2 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_001369136.1 | 100% | 100% | |
| 3 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_001369137.1 | 100% | 100% | |
| 4 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_000382.3 | 94.5% | 94.6% | 1444_1450delGCAGAATinsA;1452T>A;1455_1456ins75 |
| 5 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_001369138.1 | 94.5% | 94.6% | 1444_1450delGCAGAATinsA;1452T>A;1455_1456ins75 |
| 6 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_001369139.1 | 94.5% | 94.6% | 1444_1450delGCAGAATinsA;1452T>A;1455_1456ins75 |
| 7 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_001369146.1 | 82.1% | 80.9% | (many diffs) |
| 8 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | XM_024450651.1 | 62% | 62% | 0_1ins579 |
| 9 | human | 224 | ALDH3A2 | aldehyde dehydrogenase 3 fa... | NM_001369148.1 | 56.7% | 56.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctcgaagtc cggcgggtcc gacaggcgtt cctgtccggc cggtcgcgac 121 ctctgcggtt tcggctgcag cagctggagg ccctgcggag gatggtgcag gagcgcgaga 181 aggatatcct gacggccatc gccgccgacc tgtgcaagag tgaattcaat gtgtacagtc 241 aggaagtcat tactgtcctt ggggaaattg attttatgct tgagaatctt cctgaatggg 301 ttactgctaa accagttaag aagaacgtgc tcaccatgct ggatgaggcc tatattcagc 361 cacagcctct gggagtggtg ctgataatcg gagcttggaa ttaccccttc gttctcacca 421 ttcagccact gataggagcc atcgctgcag gaaatgctgt gattataaag ccttctgaac 481 tgagtgaaaa tacagccaag atcttggcaa agcttctccc tcagtattta gaccaggatc 541 tctatattgt tattaatggt ggtgttgagg aaaccacgga gctcctgaag cagcgatttg 601 accacatttt ctatacggga aacactgcgg ttggcaaaat tgtcatggaa gctgctgcca 661 agcatctgac ccctgtgact cttgaactgg gagggaaaag tccatgttat attgataaag 721 attgtgacct ggacattgtt tgcagacgca taacctgggg aaaatacatg aattgtggcc 781 aaacctgcat tgcacccgac tatattctct gtgaagcatc cctccaaaat caaattgtat 841 ggaagattaa ggaaacagtg aaggaatttt atggagaaaa tataaaagag tctcctgatt 901 atgaaaggat catcaatctt cgtcatttta agaggatact aagtttgctt gaaggacaaa 961 agatagcttt tggtggggag actgatgagg ccacacgcta catagcccca acagtactta 1021 ccgatgttga tcctaaaacc aaggtgatgc aagaagaaat ttttggacca attcttccaa 1081 tagtgcctgt gaaaaatgta gatgaggcca taaatttcat aaatgaacgt gaaaagccTC 1141 TGGCTCTTTA TGTATTTTCG CATAACCATA AGCTCATCAA ACGGATGATT GATGAGACAT 1201 CCAGTGGAGG TGTCACAGGC AATGACGTCA TTATGCACTT CACGCTCAAC TCTTTCCCAT 1261 TTGGAGGAGT GGGTTCCAGT GGGATGGGAG CTTATCACGG AAAACATAGT TTTGATACTT 1321 TTTCTCATCA GCGTCCCTGT TTATTAAAAA GTTTAAAGAG AGAAGGTGCT AACAAACTCA 1381 GATATCCTCC CAACAGCCAG TCAAAGGTGG ATTGGGGAAA ATTTTTTCTC TTGAAACGGT 1441 TCAACAAAGA AAAACTCGGT CTCCTGTTGC TCACTTTCCT GGGTATTGTA GCCGCTGTGC 1501 TTGTCAAGAA ATACCAAGCT GTGCTGAGGA GAAAGGCCCT GTTGATTTTT CTGGTAGTTC 1561 ACAGACTGCG TTGGTCCAGT AAGCAGAGAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACAAC 1741 CCTTCAACAG TCACCCATAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt