Construct: ORF TRCN0000470150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006021.1_s317c1
- Derived from:
- ccsbBroadEn_14431
- DNA Barcode:
- TAAAGTTAAGTTTTGGACCTCCCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAIF1 (203245)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470150
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203245 | NAIF1 | nuclear apoptosis inducing ... | NM_197956.4 | 52.1% | 51.9% | (many diffs) |
| 2 | human | 203245 | NAIF1 | nuclear apoptosis inducing ... | XR_242574.2 | 21.3% | (many diffs) | |
| 3 | human | 203245 | NAIF1 | nuclear apoptosis inducing ... | XR_428519.3 | 13.2% | (many diffs) | |
| 4 | mouse | 71254 | Naif1 | nuclear apoptosis inducing ... | NM_194335.2 | 46.3% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgtaccagcc aagaaaagga agatgaactt ctcagagcgg gaggtggaga 121 tcatcgtgga ggagctggag ctgaagaagc acctgctggt gaaccacttc aacgccgggg 181 tacccctggc cgccaagagt gcggcctggc acggcatccT GAGAAGGGTC AACGCCGTGG 241 CCACCTGCCG CAGAGAGCTG CCTGAGGTCA AGAAGAAGTG GTCTGACCTC AAGACCGAGG 301 TCCGTCGCAA GGTTGCCCAG GTCCGGGCCG CCGTGGAGGG TGGTGAGGCG CCGGGGCCCA 361 CTGAGGAGGA CGGAGCTGGG GGGCCTGGGA CAGGCGGTGG CAGTGGCGGC GGTGGCCCAG 421 CTGTAGCCCC AGTGCTGCTG ACCCCCATGC AACAACGTAT CTGCAACCTG CTGGGCGAGG 481 CCACCATCAT CAGCCTGCCC AGCACCACAG AGATCCACCC TGTGGCCCTC GGACCCTCGG 541 CCACCGCAGC CGCAGCCACG GTCACCCTGA CACAGAGTAG TGCCCCACTT TCTTGTACAA 601 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 661 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 721 GGACGATAAA GTTAAGTTTT GGACCTCCCC ACGCGTTAAG TCgacaatca acctctggat 781 tacaaaattt gtgaaagatt